Sequences from confirmation amplicon


Confirmation sequences for line 204H04 and hit At1g70960 are shown.
Line ID 204H04
Locus At1g70960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26757356
Primers used for PCR 13op - 8474, show primer details
   
Sequence Name 62-K114864-0022-204-H04C-13op
GenBank Accession LN510820 [GenBank]
Sequencing primer gene specific primer, 13op: 5'-CAAATCAGTGGTAATGAGCTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114864-0022-204-H04C-13op
GTTGGAGTCGAATTAGTCCTTATTGGAGATTGGTGAATTACAAGGGGGATATTGCCCTAA
TGAACAACATTGAGGATAGCTTGTATCACAGTCGAGAATTTGAAATGTGGGTTAGGAACG
AGGTTACTGGAAATTGGGACAGAACTAGCATTAAGATTTCTCATTGGAATGGAACTGTTG
ATGGTAAGACGTTTTATTTCAAAGGTACTATTGGAACAAAAGAACTTGCTTTCGCACCAG
ACTATTGGTTTGGGGAACAACATTTTGTGTCGTATTACGATACAGAAACAAAGAACCTCA
GAAGATTTGATATTGAAGGAATGGTTGATCAAGATGATTTTGTTCGAACCTTCTTTGATC
ATGTTGATAGTACTTGGCTTCAACGAGTTTCTTCAAGATGATTTTTCTCCACATGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 22-K114863-0022-204-H04C-8409
GenBank Accession LN496230 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114863-0022-204-H04C-8409
GTGGAGAAAATCATCTTGAAGAAACTCGTTGAAGCCAAGTACTATCAACATGATCAAAGA
AGGTTCGAACAAAATCATCTTGATCAACCATTCCTTCAATATCAAATCTTCTGAGGTTCT
TTGTTTCTGTATCGTAATACGACACAAAATGTTGTTCCCCAAACCAATAGTCTGGTGCGA
AAGCAAGTTCTTTTGTTCCAATAGTACCTTTGAAATAAAACGTCTTACCATCAACAGTTC
CATTCCAATGAGAAATCTTAATGCTAGTTCTGTCCCAATTTCCAGTAACCTCGTTCCTAA
CCCACATTTCAAATTCTCGACTGTGATACAAGCTATCCTCAATGTTGTTCATTAGGGCAA
TATCCCCCTTGTAATTCACCAATCTCCAATAAGGACTAATTTCGACTTCCAACTCAATGA
CACTAAAATCTTCAGAATTCACGTTGAAGCTCATTACACCTGATTTG

Last Updated on 06.12.2018 16:11