Sequences from confirmation amplicon


Confirmation sequences for line 205G10 and hit At2g45930 are shown.
Line ID 205G10
Locus At2g45930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18902566
Primers used for PCR 14sj - 8474, show primer details
   
Sequence Name 50-K116453-0022-205-G10C-14sj
GenBank Accession LN507289 [GenBank]
Sequencing primer gene specific primer, 14sj: 5'-GATCTCAAGTCCTAAACAATTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K116453-0022-205-G10C-14sj
CAGTCATTGCAGCGTTAGTGATGAGGCGTAATTGAGTAATATTAGGTTTACCCATGGGAT
ACATGGGATGCAAAGGAATGATTTTGGGATTTGATTTGGAGCCACAAGGATTGCAGTAGT
CGCTAACATAAACGGCTTTATGTCTACCATCACCTGTCTCCACATTGAAAACTCCCCATC
CGTGGGACATTCCAACCAGACTCGTATTACGCAACTCCATGGGAAAGGCCTTGTCTCTGA
CAATAATTTCATCTTCCTTGACCGGGTCAAAATAGTGTATAGTGCTTGTTACATCAATAT
CATCCAAACAACTTCCATAATGTATCAGACTAAACATCAAGTAAGGGTTGGTGGTGGTGT
ATAATTGACCATCATATTGTATAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 17-K116452-0022-205-G10C-8409
GenBank Accession LN494012 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116452-0022-205-G10C-8409
GCATTACATTGAATATATACATATGATGGTCAATTATACACCACCACCAACCCTTACTTG
ATGTTTAGTCTGATACATTATGGAAGTTGTTTGGATGATATTGATGTAACAAGCACTATA
CACTATTTTGACCCGGTCAAGGAAGATGAAATTATTGTCAGAGACAAGGCCTTTCCCATG
GAGTTGCGTAATACGAGTCTGGTTGGAATGTCCCACGGATGGGGAGTTTTCAATGTGGAG
ACAGGTGATGGTAGACATAAAGCCGTTTATGTTAGCGACTACTGCAATCCTTGTGGCTCC
AAATCAAATCCCAAAATCATTCCTTTGCATCCCATGTATCCCATGGGTAAACCTAATATT
ACTCAATTACGCCTCATCACTAACGCTGCAATGACTTGTTCACCAGATCAAAGTAAAGAT
TTTGTAGTGGCTGCCAATTGTTTAGATTTTGAGATC

Last Updated on 06.12.2018 16:11