Sequences from confirmation amplicon


Confirmation sequences for line 205H07 and hit At2g42270 are shown.
Line ID 205H07
Locus At2g42270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17608221
Primers used for PCR 09uj - 8474, show primer details
   
Sequence Name 58-K117671-0022-205-H07C-09uj
GenBank Accession LN509817 [GenBank]
Sequencing primer gene specific primer, 09uj: 5'-TAAGATCCAATAATGTCTCCCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117671-0022-205-H07C-09uj
CAGCCCCTTTCGAACTTTTTCTCCCATCACGAAACTGCTCCTTGGCAATAGCCTCAAGAG
GTGCTATATAGACAACACGCATTGCAGAATCAGGTCCTTCGAGATGATTCCTTAATATAG
CAAACTCCGCACATATAGTCTTCCCACTTCCTGTTGGAGCAGCAACCACTACATTGTCAC
TTGTGTTATACAAAACAGTAAAGACCTGAGTCTGCACTGGATTGAAATGCTTGAAATCCT
GATAAAGAGTTTCATAAGAAGGATTTCTTAGGGCCATCACCGGGAGGGGCTGCAAATCCA
GTAGCTCTGTAGGTGGTGGGTATTTTTCTGGAAGAATAAGATGCCTGAAAGATACAGGTA
ACACAGTCGGCGAGTCAAGCCATTTGTCTGAACCATGGCTCAGAGATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTAT
TAT
   
Sequence Name 10-K117670-0022-205-H07C-8409
GenBank Accession LN490845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K117670-0022-205-H07C-8409
CATTTACATTGAATCTCTGAGCCATGGTTCGACAAATGGCTTGACTCGCCGACTGTGTTA
CCTGTATCTTTCAGGCATCTTATTCTTCCAGAAAAATACCCACCACCTACAGAGCTACTG
GATTTGCAGCCCCTCCCGGTGATGGCCCTAAGAAATCCTTCTTATGAAACTCTTTATCAG
GATTTCAAGCATTTCAATCCAGTGCAGACTCAGGTCTTTACTGTTTTGTATAACACAAGT
GACAATGTAGTGGTTGCTGCTCCAACAGGAAGTGGGAAGACTATATGTGCGGAGTTTGCT
ATATTAAGGAATCATCTCGAAGGACCTGATTCTGCAATGCGTGTTGTCTATATAGCACCT
CTTGAGGCTATTGCCAAGGAGCAGTTTCGTGATTGGGAGAAAAAGTTCGGAAAGGGGCTT
GGTTTACGGGTTGTTGAGTTAACTGGGGAGACATTATGGGATCTT

Last Updated on 06.12.2018 16:11