Sequences from confirmation amplicon


Confirmation sequences for line 206D06 and hit At2g35770 are shown.
Line ID 206D06
Locus At2g35770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15034259
Primers used for PCR 07po - 8474, show primer details
   
Sequence Name 43-K115714-0022-206-D06C-07po
GenBank Accession LN504951 [GenBank]
Sequencing primer gene specific primer, 07po: 5'-GAGATTTGTTTATGAGCGAAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115714-0022-206-D06C-07po
TACTACTTTATTTTAATTAATATTAAAAGAAAAAGATGTATATTTGTCTTCTAATACGTA
ACAACTACACACAAAACAGAAACAAATACCACCTATCAATAAAAAACGCAAATAATGATG
TTTACAAAATGTACCTCATGCAAATAAGTGGAAAAAAAATTAAGAGGGAGAGGAGGGAAG
AGAGTGGTTGGCTAGGAAGTGGGTGAAGAGGAAAAGAGCGAGACGAGGCTGAGACAATGG
CTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGTATTATA
   
Sequence Name 03-K115713-0022-206-D06C-8409
GenBank Accession LN487623 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115713-0022-206-D06C-8409
ATTTACAATTGAACCATTGTCTCAGCCTCGTCTCGCTCTTTTCCTCTTCACCCACTTCCT
AGCCAACCACTCTCTTCCCTCCTCTCCCTCTTAATTTTTTTTCCACTTATTTGCATGAGG
TACATTTTGTAAACATCATTATTTGCGTTTTTTATTGATAGGTGGTATTTGTTTCTGTTT
TGTGTGTAGTTGTTACGTATTAGAAGACAAATATACATCTTTTTCTTTTAATATTAATTA
AAAATAAAGTAGTACATGGAGATCAAACTTGATTGGCTGAAAATGTTTTCCAAACCTCTT
CGCTCATAAACAAATCTCA

Last Updated on 06.12.2018 16:11