Sequences from confirmation amplicon


Confirmation sequences for line 206F05 and hit At3g25420 are shown.
Line ID 206F05
Locus At3g25420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9220491
Primers used for PCR 13fm - 8474, show primer details
   
Sequence Name 24-K111117-0022-206-F05C-13fm
GenBank Accession LN497045 [GenBank]
Sequencing primer gene specific primer, 13fm: 5'-CATGATTGCCACTTTGAAAACTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111117-0022-206-F05C-13fm
ATCTACATTTGGATACATTGTATATTAGGATCATATATATACATAAATAGTTTAAAAAGT
TGTAAGAACAAGGGTATTTAATTTTCATCATTAGACACAAATAGTTCATTTGATCAAATT
AAATGTATAGTACCAATTGTAATACATTTGATGATTCTCCACAATTTTAGAAACTGAAAA
CTTGTAATGGATGCAGGACGATACAGTTGCGACCAAATGGCTAAACGATCCAGCTGTAAG
GAAAGCGGTTCATGCTAAAGAGGTCTCTATTCAATTCATCATATTTCTCTCAATCAGTAT
TTGTAATAAGTTGATGATGGACATTGGACACCATTTTAACATCTTGGACCTTTTTTCGAA
ACGTCGATATAGGAAAAAGCGATTGGAAATTGGGAGTTATGTTCAAGCAACCTCGAATAT
AGGCACGACACCGGTAGCATGATCGAATACCATAGAAACCTCACTCTTAGTGGCTTTCGA
GCTCTCATTTTCAGGTGCACATATNTNGAAATAAATCANAGGTAACTAANAGTAAAACGA
ATGAAAATATAANATATAACANAAGTTAAATTTTTTGCAGTGGAGATCATGATATGTGTG
TACCATATACTGGCTCTGAAGCATGGA
   
Sequence Name 08-K111116-0022-206-F05C-8409
GenBank Accession LN489786 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111116-0022-206-F05C-8409
CACACATCCAAGTCAAATGTAGACATGACATGGGAACTATTTAAAATTGGTTATTTGTAT
CCCATTGCCTTAGTCCATGCTTCAGAGCCAGTATATGGTACACACATATCATGATCTCCA
CTGCAAAAAATTTAACTTTTGTTATATTTTATATTTTCATTCGTTTTACTTTTAGTTACC
TTTGATTTATTTCTATATATGTGCACCTGAAAATGAGAGCTCGAAAGCCACTAAGAGTGA
GGTTTCTATGGTATTCGATCATGCTACCGGTGTCGTGCCTATATTCGAGGTTGCTTGAAC
ATAACTCCCAATTTCCAATCGCTTTTTCCTATATCGACGTTTCGAAAAAAGGTCCAAGAT
GTTAAAATGGTGTCCAATGTCCATCATCAACTTATTACAAATACTGATTGAGAGAAATAT
GATGAATTGAATAGAGACCTCTTTAGCATGAACCGCTTTCCTTACAGCTGGATCGTTTAG
CCATTTGGTCGCAACTGTATCGTCCTGCATCCATTACAAGTTTTCAGTTTCTAAAATTGT
GGAGAATCATCAAATGTATTACAATTGGTACTATACATTTAATTTGATCAAATGAACTAT
TTGTGTCTAATGATGAAAATTAAATACCCTTGTTCTTACAACTTTTTAAACTATTTATGT
ATATATATGATCCTAATATACAATGTATCCAAATGTAGATATGTAAATTCGAGTGTAACC
TAGTGATAATTAGTTTTC

Last Updated on 06.12.2018 16:11