Sequences from confirmation amplicon


Confirmation sequences for line 206F10 and hit At1g15400 are shown.
Line ID 206F10
Locus At1g15400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5296736
Primers used for PCR AP15 - 8474, show primer details
   
Sequence Name 06-K106540-0022-206-F10C-8409
GenBank Accession LN488825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106540-0022-206-F10C-8409
TCGGACTGAGCCTTTACATTGAATATATTCACAAATCGGGAAACAACGAGCAGAAAGACG
AAAGTCAACGCGACGAACAACCTAAGCCTATGTCGGAAAGCTCTGAACAGGTCAAACCAA
TCGATGAGAAAGACAAGCTTAGACCGATTAAAACCGGTGGAGGAGCACCAGGAGGAATAG
AGAGGAGTCGATCTAACGGTGGAGGAGCTCAACGACATCATAGAACAACCGGAAGAGTTT
CTCCGGCAGTGGACCCGCCGTCTCCGAGAATTTCGTCATGCGGTTGTTGCTCTGCGTTTG
GAAAGAATCCGCCGGGGAAGAAAGTTAATCCGAGGAAAAGGCCGCCGAAACGTAGATCGA
GGTAGGCGTCAGGATTAATAATATTCATGAGGAAAAAATAGTATACACTGTCTTTCATCT
TTCTTCTTCTTCTTTTTTGTATGTGTGTGTTCTTTGTTATAAAAAACTTTAAATTTGATT
TTTCGATTTGATCAACAGACTCTGGTTATTTGTAAATTATCTGTCTTTCTTTGTTTTCGG
ACAATTTCCCATTTCTGTACAAAATCAAACTTTCCGATTTGGTGATTCTTCTTCCTTCTT
GGCGAATTATCTGATTAAGTGATTTCAGTATAAAACATTTTGAGAAAAATCAAACAACGT
TCAAGTCCCTTCCGATGGCTCACTGACTAATCCTCTAGGGTTTCG
   
Sequence Name 22-K106541-0022-206-F10C-AP15
GenBank Accession LN496127 [GenBank]
Sequencing primer gene specific primer, AP15: 5'-TTCGAAACCCTAGAGGATTAGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106541-0022-206-F10C-AP15
TTTGATTTTTCTCAAATGTTTTATACTGAAATCACTTAATCAGATAATTCGCCAAGAAGG
AAGAAGAATCACCAAATCGGAAAGTTTGATTTTGTACAGAAATGGGAAATTGTCCGAAAA
CAAAGAAAGACAGATAATTTACAAATAACCAGAGTCTGTTGATCAAATCGAAAAATCAAA
TTTAAAGTTTTTTATAACAAAGAACACACACATACAAAAAAGAAGAAGAAGAAAGATGAA
AGACAGTGTATACTATTTTTTCCTCATGAATATTATTAATCCTGACGCCTACCTCGATCT
ACGTTTCGGCGGCCTTTTCCTCGGATTAACTTTCTTCCCCGGCGGATTCTTTCCAAACGC
AGAGCAACAACCGCATGACGAAATTCTCGGAGACGGCGGGTCCACTGCCGGAGAAACTCT
TCCGGTTGTTCTATGATGTCGTTGAGCTCCTCCACCGTTATATCGACTCCTCTCTATTCC
TCCTGGTGCTCCTCCAAAGGTTTTAATCGGTCTAAGCTTGTCTTTCTCATCGATTGGTTT
GACCTGTTCAGAGCTTTCCGACATAGGCTTAGGTTGTTCGTCGCGTTGACTTTCGTCTTT
CTGCTCGTTGTTTCCCGATTTGTTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
Primers used for PCR 35xg - 8474, show primer details
   
Sequence Name 38-K119006-0022-206-F10C-35xg
GenBank Accession LN503167 [GenBank]
Sequencing primer gene specific primer, 35xg: 5'-TATTTATGGATGGGCCTGAGAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K119006-0022-206-F10C-35xg
GCCAATTCTTTTCTCAATCTTCACATTGGTGAGTTGTGCCAACTCAGACGGGGTTATTAA
TTTGTGTTATGCACATTACTTTAATCCAAAGAATACAGTTTTGCTAAACCCACCACATGC
GTATGTTTTAGTTAGTGGTGATCCTGAGAAGAAACAGACGATATAAGAAGGTGAATTATT
GAACAAAAGTTATACCATATTATAGTACAATGATGTTGACTTTGAATACAAATTGGTAAC
CGAATTACTTTGACTCACTTTCACCACTTTGTATTGTTTTTCCTTTCAAGTTTTTATAAA
GAGAGATTTGGCATTTTAATAACATCCTCACCTAATAGATTTGGTTTACCGAAATGGGGA
AGAAGGAAATAGTCTATTTGGTTTACCGAAATGGGGAAGAAGGAAATATTAAATTAGTGT
GTCCTATAAGTCAATTCTCCTGTTCATTTCTAATCTATTATCTAATCCTTGCAATTTGCT
GATAAAACAATAGTAATTACAAAAAGTCTTACCCCTTTTGAGTTTAAAGAAACCAAAAGT
TTGGAATAAAATAACAAATATGGATATAAGAGGACTTATGTGCCTATGTGGTGTTGAGAA
TCCGATTAAAGATGTGAAAACAAACAAAAAATTAACATGTAATTCTCTGCCTTAATCATC
AATAACAGTAATGGTTTTTACTTTTTTTTTTTTATAACAATA
   
Sequence Name 08-K119005-0022-206-F10C-8409
GenBank Accession LN489952 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119005-0022-206-F10C-8409
TTACATTGAATATGGATCATATTGAATGATCCATATTAATCATCATACTCAGGATATTCA
ATTGCTCATTGCGGATTAACGATATCATTCAACCGNGATTATTCAAACATTAATTGTAAT
AAAAAAAAAAAAGTAAAACCCTTTCCGGTTTTGGATGATTAGGGCAAAAAATTCCAGGTA
AATTTTTGGTTGGTTTCCCCACCTTAAACCGGATTCCCAACCCCACATGGGCACATAAGC
CCCCTTATACCCATATTGGTTATTTTATCCCAAACTTTGGGTTTCTTTAAACTCAAAGGG
GGAAAAACTTTTTGAAATTCCTATGGTTTTACCACCAATTTGCAGGGATTAGATAATAGA
TTAAAATTGAACGGGAAATTTGCCTTATGGGACCCCCTAATTTAATTTTCCCTTCTCCCC
CATTTCGGAAAACCAAAAAAACTATTTCCTTCTTCCCCATTTCGGAAAACCAAATCTTTT
AGGGGAGGATGTTATTAAAAGGCCAAATCTCTCTTTATAAAACCTTGAAGGGAAAACCAA
TCCAAGGGGGGGAAAGTGAGTCAAAGTATTTCGGTTCCCATTTTGTATTCAAAGCCAACA
TCATGGAACTAAAATAGGGTATAACTTTTGTTCAATAATCCACCTTCTTATACCGTCGGT
TTCTCCCCAGGATCCC

Last Updated on 06.12.2018 16:11