Sequences from confirmation amplicon


Confirmation sequences for line 207C02 and hit At4g39770 are shown.
Line ID 207C02
Locus At4g39770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18449635
Primers used for PCR MS23 - 8474, show primer details
   
Sequence Name 21-K016405-022-207-C02C-8409
GenBank Accession LN495450 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K016405-022-207-C02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAAATGACGAGAGAAATGGTTTATATCAA
TAGTATTAATTAAAATTAGTATTGGATTGTGTAGGATACGCTAATTGTACCGACGTTTTC
CCTCTTTATATCGGAGATGATCGCACCGACGAAGATGCATTCAAGGTATATACAGAATTT
AGTAATCATTTATTAGATTAAATTAAATAATTTATGTTCAATCATCTAAGTAAAATTGAT
TTAATTATTGTTATAGGTATTGAGAGAACGAAGACAAGGTCTTGGCATTCTTGTATCTAA
ATTTCCAAAGGAGACTAGCGCGTCTTATTCACTGCAAGAACCCGATGAGGTAAATTGATC
AAGACAAGCACAACTTCGATCTAATTTAGAAACTTCCCATTTTAGAATTATTGTTATATA
TGTCCACTGTACTCTGCTGATAATAATACTATACGTTTTCTTAATTATTAACAGGTTATG
GAGTTCTTGCAACGTTTAGTGGAATGGAAACAATTGAGATCT
   
Sequence Name 22-K016405-022-207-C02C-MS23
GenBank Accession LN495893 [GenBank]
Sequencing primer gene specific primer, MS23: 5'-ATCATGCTCCAGATCTCAATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K016405-022-207-C02C-ms23
CACTAAACGTTGCAAGAACTCCATAACCTGTTAATAATTAAGAAAACGTATAGTATTATT
ATCAGCAGAGTACAGTGGACATATATAACAATAATTCTAAAATGGGAAGTTTCTAAATTA
GATCGAAGTTGTGCTTGTCTTGATCAATTTACCTCATCGGGTTCTTGCAGTGAATAAGAC
GCGCTAGTCTCCTTTGGAAATTTAGATACAAGAATGCCAAGACCTTGTCTTCGTTCTCTC
AATACCTATAACAATAATTAAATCAATTTTACTTAGATGATTGAACATAAATTATTTAAT
TTAATCTAATAAATGATTACTAAATTCTGTATATACCTTGAATGCATCTTCGTCGGTGCG
ATCATCTCCGATATAAAGAGGGAAAACGTCGGTACAATTAGCGTATCCTACACAATCCAA
TACTAATTTTAATTAATACTATTGATATAAACCATTTCTCTCGTCATTTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA

Last Updated on 06.12.2018 16:11