Sequences from confirmation amplicon


Confirmation sequences for line 207E06 and hit At2g13650 are shown.
Line ID 207E06
Locus At2g13650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5687740
Primers used for PCR GE35 - 8474, show primer details
   
Sequence Name 55-K100940-0022-207-E06C-8409
GenBank Accession LN508748 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K100940-0022-207-E06C-8409
GAGCCATTTACAATTGAATATATCCTGAAGAGTATGCTTGCTGAGTTCTGCAAACTGGTC
GGTACATTGAAAAGAACGATCCCCGCGATGGATAGAGGTATCTTGTTTAGAGATCCCACC
AGGCTATGATTTTGAATAAAGCGAAAGATGTATCTAGTTAGGACTGAACCAACAAAGAAG
CAAACATAGGAAAGGGATATATGGAGAAGGCTAGGGGACAGCAACACTCTCTTAAGCAAA
GTTTTGAAATTGCTCTATCTCTCTCTAATTTGCTACCTGTATGTTGTTGCTCCAGTTTGA
TGAAGAAACCACATTGAAG
   
Sequence Name 55-K100947-0022-207-E06C-GE35
GenBank Accession LN508749 [GenBank]
Sequencing primer gene specific primer, GE35: 5'-TGCCATTAGCTTTACTTCAATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K100947-0022-207-E06C-GE35
ACACACATACAGGTAGCAATTAGAGAGAGATAGAGCAATTTCAAACTTTGCTTAAGAGAG
TGTTGCTGTCCCCTAGCCTTCTCCATATATCCCTTTCCTATGTTTGCTTCTTTGTTGGTT
CAGTCCTAACTAGATACATCTTTCGCTTTATTCAAAATCATAGCCTGGTGGGATCTCTAA
ACAAGATACCTCTATCCATCGCGGGGATCGTTCTTTTCAATGTACCGACCAGTTTGCAGA
ACTCAGCAAGCATACTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11