Sequences from confirmation amplicon


Confirmation sequences for line 208B06 and hit At3g61172 are shown.
Line ID 208B06
Locus At3g61172 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22641824
Primers used for PCR 09qp - 8474, show primer details
   
Sequence Name 56-K115691-0022-208-B06C-09qp
GenBank Accession LN509197 [GenBank]
Sequencing primer gene specific primer, 09qp: 5'-ATCCTTTTCTTTTTCCAACCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K115691-0022-208-B06C-09qp
CTCACCAATGTTGTCATCTTCATTGTCTTCTCTCCTAGGTTTGTTTTACATTTCCATTCT
CTAACTATATTATAAATGACCATTAACAAATTAATCAAATAAATTTTCACGTTTTTATGC
TTTGTATATTGAAATTTGATGTTTAAAAATATTGTTTTAGGGATGATGGCGAAAGAAACG
CAAGGACAACATATATGTCATCAGATTCTGTTAAACAACAATTGTGATGGAGCCACATGC
ACAAGTCTCTGTGACAAGCAGTTGCAAGGAACTGGCCAGCAATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K115690-0022-208-B06C-8409
GenBank Accession LN489868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115690-0022-208-B06C-8409
TTGAATTGCTGGCCAGTTCCTTNAACTGCTTGTCACAGAGACTTGTGCATGTGGCTCCAT
CACAATTGTTGTTTAACAGAATCTGATGACATATATGTTGTCCTTGCGTTTCTTTCGCCA
TCATCCCTAAAACAATATTTTTAAACATCAAATTTCAATATACAAAGCATAAAAACGTGA
AAATTTATTTGATTAATTTGTTAATGGTCATTTATAATATAGTTAGAGAATGGAAATGTA
AAACAAACCTAGGAAGAAGACAATGAAGATGACAACATTGGTGAGTTTACTCATCTTTTT
GATGATATAATAGGATTTGGTTGGAAAGNAAAAAGGATA

Last Updated on 06.12.2018 16:11