Sequences from confirmation amplicon


Confirmation sequences for line 208D03 and hit At3g16180 are shown.
Line ID 208D03
Locus At3g16180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5484302
Primers used for PCR 13de - 8474, show primer details
   
Sequence Name 48-K109414-0022-208-D03C-13de
GenBank Accession LN506525 [GenBank]
Sequencing primer gene specific primer, 13de: 5'-AGGACAAACTGTCTTGTTCATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K109414-0022-208-D03C-13de
AGCTTTTCTCTCAGATTCATATTTGGGTCGCTTTCTCACCATTGTCATTGCTTCTCTCTC
CAGTCTCCTGGTAATTTCCGTCCCCTATTTAACCGGTTTACGCTTCTTCTTTCTCCCGGT
TTACTCCGGTTCAATTTTTTAGCCACTTCTTTGACTCAACATTTTTCACCACCTAGTTAG
CCTTTGGTTTGGAAATGCTTTGTTTATGTTTACTGTCAGTAAAGGTTGAGTTTTATTAAA
ATTGGGAATCTAAACCGAAAGTTTCGGTTTAAGTTGATTTGGGTGAAATATCGTGCTTGT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGC
   
Sequence Name 32-K109413-0022-208-D03C-8409
GenBank Accession LN500620 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K109413-0022-208-D03C-8409
GCCATTTACATTGAATACAGCACGATATTTCACCCAAATCAACTTAAACCGAAACTTTCG
GTTTAGATTCCCAATTTTAATAAAACTCAACCTTTACTGACAGTAAACATAAACAAAGCA
TTTCCAAACCAAAGGCTAACTAGGTGGTGAAAAATGTTGAGTCAAAGAAGTGGCTAAAAA
ATTGAACCGGAGTAAACCGGGAGAAAGAAGAAGCGTAAACCGGTTAAATAGGGGACGGAA
ATTACCAGGAGACTGGAGAGAGAAGCAATGACAATGGTGAGAAAGCGACCCAAATATGAA
TCTGAGAGAAAAGCTCCAACGAGAGGCATGAAATTAGTAGCAGCAACCCACATGAACAAG
ACAGTTTGTCC

Last Updated on 06.12.2018 16:11