Sequences from confirmation amplicon


Confirmation sequences for line 208E01 and hit At1g51780 are shown.
Line ID 208E01
Locus At1g51780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19206159
Primers used for PCR 11lg - 8474, show primer details
   
Sequence Name 44-K113934-0022-208-E01C-11lg
GenBank Accession LN505256 [GenBank]
Sequencing primer gene specific primer, 11lg: 5'-CAAGGCCTTTACTTGTGACCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113934-0022-208-E01C-11lg
GACTCCGTAACTAATAGAACTTGACACTGCCGTTTATTTTATTGATTGACAACTGAGAAT
TACAGGGAATAACTGTTACCATGTAACCATGGTTGACATTTTCGATAGGTACATGAGATG
AAGAAGAGAGATCCAAAGTTGGCATCAAAGTTCATCTTTATTTGGCGATGAAGATGAGTC
AAGAAGATATCTTGTGGCCAAAGAAGCAAGAAGGGAAGCACCATAAGGGAGTAATTCTTC
ATTAACTTCAAAAAACGGAGAGTGAGGGTTCGCCATTGGTGAATGAGATTTGTTCTGCAT
CCCAACAAAGCTAAAATGTCCCGGGATTGCTTGTTGGTAGAAAGCAAAATCCTCTGATAC
CATCACTGGTAGCGTCTCAACATAATTCTCAATCCCTAACATATCCACTGAAACGTTCTT
GTAAAACAGGTGCAAGGTTTTATTGTTCACAGTCGGCGGGAAAGGAGGAGAGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K113933-0022-208-E01C-8409
GenBank Accession LN490266 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113933-0022-208-E01C-8409
AACCTTGCACCTGTTTTACAAGAACGTTTCAGTGGATATGTTAGGGATTGAGAATTATGT
TGAGACGCTACCAGTGATGGTATCAGAGGATTTTGCTTTCTACCAACAAGCAATCCCGGG
ACATTTTAGCTTTGTTGGGATGCAGAACAAATCTCATTCACCAATGGCGAACCCTCACTC
TCCGTTTTTTGAAGTTAATGAAGAATTACTCCCTTATGGTGCTTCCCTTCTTGCTTCTTT
GGCCACAAGATATCTTCTTGACTCATCTTCATCGCCAAATAAAGATGAACTTTGATGCCA
ACTTTGGATCTCTCTTCTTCATCTCATGTACCTATCGAAAATGTCAACCATGGTTACATG
GTAACAGTTATTCCCTGTAATTCTCAGTTGTCAATCAATAAAATAAACGGCAGTGTCAAG
TTCTATTAGTTACGGAGTCTAAGTCACCAAAGAGAAACAGAGGAGGACAGAAAACTGGAA
GTTCCTTAGGTCACAAGTAAAGGCCTTG

Last Updated on 06.12.2018 16:11