Sequences from confirmation amplicon


Confirmation sequences for line 208H08 and hit At1g30900 are shown.
Line ID 208H08
Locus At1g30900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10999960
Primers used for PCR 32jx - 8474, show primer details
   
Sequence Name 72-K113379-0022-208-H08C-32jx
GenBank Accession LN512757 [GenBank]
Sequencing primer gene specific primer, 32jx: 5'-TTGAAACAAATGAATGTCTCGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113379-0022-208-H08C-32jx
GAGTCCATGTACAGCTTGCAGGTACTTAGAACCGATGAACTTAAGGGACTACATTTTTAT
AGATATTTGGTTAGTTTTATACCTAAAAATGGATTCTATCAGGACACATTTAGAGGAAGA
GTCTGTGAATGCCCTGTTGTGAATGGTGTACAGTATAAAGGAGATGGATATACATCATGT
GAACGTACGTTCTTCAACTTCTACATTGATATCTTGTAGAAACACTTCATTATAAACATT
TTGTGTTCTGACTTGAATGTTATAACAGCTTATGGCCCTGCAAGATGCTCGATTAACCAA
GGAGGTTGCTGGTCTGAAACCAAAAAGGGCTTAACTTTCTCGGCTTGCTCGGTTAGTTAC
TCTACTGTGTTATGGCCTTTTCAGTAATAATGCCATAATGTTTGAAACTTACAAATTTTC
TCCAATTGTTGAGTTCAGAACTTGGAGACATCGGGATGTCGCTGCCCTCCAGGGTTTAAA
GGAGATGGTCTTAAATGTGAAGGTGAAAACTTTTATTTTCTCTAGAATTCAAAATCTCTC
ATCATGTTTTCTTAAACAGCTTGTCAGCCCAATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 34-K113377-0022-208-H08C-8409
GenBank Accession LN501485 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113377-0022-208-H08C-8409
TCGGATGAGCCTTTACATTGATTGGGCTGACAGCTGTTAAGAAAACATGATGAGAGATTT
TGAATTCTAGAGAAAATAAAAGTTTTCACCTTCACATTTAAGACCATCTCCTTTAAACCC
TGGAGGGCAGCGACATCCCGATGTCTCCAAGTTCTGAACTCAACAATTGGAGAAAATTTG
TAAGTTTCAAACATTATGGCATTATTACTGAAAAGGCCATAACACAGTAGAGTAACTAAC
CGAGCAAGCCGAGAAAGTTAAGCCCTTTTTGGTTTCAGACCAGCAACCTCCTTGGTTAAT
CGAGCATCTTGCAGGGCCATAAGCTGTTATAACATTCAAGTCAGAACACAAAATGTTTAT
AATGAAGTGTTTCTACAAGATATCAATGTAGAAGTTGAAGAACGTACGTTCACATGATGT
ATATCCATCTCCTTTATACTGTACACCATTCACAACAGGGCATTCACAGACTCTTCCTCT
AAATGTGTCCTGATAGAATCCATTTTTAGGTATAAAACTAACCAAATATCTATAAAAATG
TAGTCCCTTAAGTTCATCGGTTCTAAGTACCTTGCAAGCTGTTACATTGGACTTCTTGTC
CTCCCAACACCCTCCATTTGCTTCGAGACAT

Last Updated on 06.12.2018 16:11