Sequences from confirmation amplicon


Confirmation sequences for line 209G07 and hit At4g30935 are shown.
Line ID 209G07
Locus At4g30935 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15054054
Primers used for PCR IS22 - 8474, show primer details
   
Sequence Name 05-K017254-022-209-G07C-IS22
GenBank Accession LN488143 [GenBank]
Sequencing primer gene specific primer, IS22: 5'-CGCACAGTCTCATCATGTAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K017254-022-209-G07C-IS22
ATTTCCTCGTCTCTGTATTGACTCAGTCCGTCCTTCTCCGGCTCCACTTTCTCGCTCTTC
TCCACCGTGTATGTCTTAGCTTCGTCGATACCAGTGTCTTCTTCCATGGACGATTCTCTC
TTGTCCTCTTTTTCTGTATCTCGTGGGTTTTGTCTGAAGAGAGTAAAAGAAAGAAACGAA
CAAATTCTCTAGGCAGACAAAAAGGTAGCCGCTTTCCGACCGACTGATCTCCGGAAGAAA
ACGCTGCGTTTTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCACGTCCCAATGG
   
Sequence Name 06-K017254-022-209-G07C-8409
GenBank Accession LN488586 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K017254-022-209-G07C-8409
CGGACATGAAGCCATTTACAATTGAATATATCAAAACGCAGCGTTTTCTTCCGGAGATCA
GTCGGTCGGAAAGCGGCTACCTTTTTGTCTGCCTAGAGAATTTGTTCGTTTCTTTCTTTT
ACTCTCTTCAGACAAAACCCACGAGATACAGAAAAAGAGGACAAGAGAGAATCGTCCATG
GAAGAAGACACTGGTATCGACGAAGCTAAGACATACACGGTGGAGAAGAGCGAGAAAGTG
GAGCCGGAGAAGGACGGACTGAGTCAATTCAGAGACGAGGAAAAATCACTTGGTGCGGAT
ATGGAAGATTTACATGATGAGACTGTGC

Last Updated on 06.12.2018 16:11