Sequences from confirmation amplicon


Confirmation sequences for line 210G09 and hit At1g34180 are shown.
Line ID 210G09
Locus At1g34180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12450749
Primers used for PCR 16fm - 8474, show primer details
   
Sequence Name 23-K111117-0022-210-G09C-16fm
GenBank Accession LN496608 [GenBank]
Sequencing primer gene specific primer, 16fm: 5'-GAGTTCTTGAACCATGGTGAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K111117-0022-210-G09C-16fm
TGACATATCTGTTTTTCAGGGAACTTCTACAGATCTGTCTTGTCTGAGTAATTTTACTAA
TAACACATCAGGCCAAAGACAGCAATTACTTTATGAACAGTTCCAGTACCAGACACCTGA
GAACCAGCTTAATAACTACATGCATCCTAGTACCACTCTTAATCAGTTCACTGACAATAT
GTGGTTTAAAGATGATCAGGCTGCTCTCTATGTTCAACCACCACAATCTTCTTCTGGAGC
ATTCACTTCACAGTCAACAGGTACTTAAAAAGTCTTTAAACTCACCCTCATCTCAGAGCA
TTCCCTTATGTTGTCTGCCAAGTTATATTTCCCACGAAGCATTAGTTTTTGAATTTAACC
AAGACATGTATATATTATACTCTGTTTTCTTTTTGTTGATATCAAACCCTCTTGTTAAGA
TCTTGAAACATAATAACCTAGGGCCTTTTTTACTGATACTTTGTGAATACGGACATATGG
ATATAGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTC
   
Sequence Name 07-K111116-0022-210-G09C-8409
GenBank Accession LN489330 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K111116-0022-210-G09C-8409
TCATTGAATATTCCTCTATATCATATGTCCGTATTCACAAAGTATCAGTAAAAAAGGCCC
TAGGTTATTATGTTTCAAGATCTTAACAAGAGGGTTTGATATCAACAAAAAGAAAACAGA
GTATAATATATACATGTCTTGGTTAAATTCAAAAACTAATGCTTCGTGGGAAATATAACT
TGGCAGACAACATAAGGGAATGCTCTGAGATGAGGGTGAGTTTAAAGACTTTTTAAGTAC
CTGTTGACTGTGAAGTGAATGCTCCAGAAGAAGATTGTGGTGGTTGAACATAGAGAGCAG
CCTGATCATCTTTAAACCACATATTGTCAGTGAACTGATTAAGAGTGGTACTAGGATGCA
TGTAGTTATTAAGCTGGTTCTCAGGTGTCTGGTACTGGAACTGTTCATAAAGTAATTGCT
GTCTTTGGCCTGATGTGTTATTAGTAAAATTACTCAGACAAGACAGATCTGTAGAAGTTC
CCTGAAAAACAGATATGTCATTGAACAATTGGTCGTATTCATTAACATCACCAAATTCAC
CATGGTTCAAGAACT

Last Updated on 06.12.2018 16:11