Sequences from confirmation amplicon


Confirmation sequences for line 212A03 and hit At2g13500 are shown.
Line ID 212A03
Locus At2g13500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5628730
Primers used for PCR KT71 - 8474, show primer details
   
Sequence Name 58-K100815-0022-212-A03C-8409
GenBank Accession LN509640 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K100815-0022-212-A03C-8409
AGATTCTTGTTGGCACTCGTGGAGGTGGATGTAAAGGAACCTGAAGAGAGCTATCAGTCA
TCTGAGATTGAGAGTTAAAGCTTGACCTCAAAACACTATCTCTGAAAACAGCTCTTTCCC
TGTCAATGCTCTGAAAACGTTGGCGATAAGGATAATAGTTCAAGTTATGGGTAAACCTAA
AACAACTATAGATGCGCCTAAGTCTCTCATACTGAAACCGAATTAAAGCCGTTTCTCCTG
ACTCAAAGGTGATTCTCTGAAAGAACCTTAAACGATCAGTAATACCAAAACGAACTCTAA
CTCGGATATAAGCAATCTGTGTAGAAGTGGCATCATGAAAGT
   
Sequence Name 58-K100827-0022-212-A03C-KT71
GenBank Accession LN509642 [GenBank]
Sequencing primer gene specific primer, KT71: 5'-GACTTTCATGATGCCACTTCTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K100827-0022-212-A03C-KT71
GTTTTGGTATACTGATCGTTTAGGTTCTTTCAGAGAATCACCTTTGAGTCAGGAGAAACG
GCTTTAATTCGGTTTCAGTATGAGAGACTTAGGCGCATCTATAGTTGTTTTAGGTTTACC
CATAACTTGAACTATTATCCTTATCGCCAACGTTTTCAGAGCATTGACAGGGAAAGAGCT
GTTTTCAGAGATAGTGTTTTGAGGTCAAGCTTTAACTCTCAATCTCAGATGACTGATAGC
TCTCTTCAGGTTCCTTTACATCCACCTCCACGAGTGCCAACAAGAATCTACATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG

Last Updated on 06.12.2018 16:11