Sequences from confirmation amplicon


Confirmation sequences for line 212B05 and hit At2g47970 are shown.
Line ID 212B05
Locus At2g47970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19630032
Primers used for PCR 12gp - 8474, show primer details
   
Sequence Name 27-K112307-0022-212-B05C-12gp
GenBank Accession LN498456 [GenBank]
Sequencing primer gene specific primer, 12gp: 5'-AAGAAAGAAGGAAGACGATGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112307-0022-212-B05C-12gp
GTCACAGCGGAGGGAGCTCATATCACTGTTTCCCAGCTCAAAACCTTGATCGCGGATCAG
CTTCAGATCCCTCTCCATAAGCAGACCTTATCCACCAATCGTGATCTCCTCCTCGCCAAA
ACTCCCGCTGATCTACTCGCCTTCACCGACCTCACGGACCCAAACCTCCCCCTCTCCTCT
CTCAACCTCGGCCATGGCTCTATGCTCTACCTCGCCTACGATGGCGAGCGCTCCATTCCT
GGAGCTCCTCCCGTTACTCCTGCCGGATCCTTCGGGAGGAAGATGACCGTCGATGACTTG
ATCGCCCGCCAGATGCGCGTCACTCGTCAGGAGACGTCTCACTGCGACTCCGTCTCCTTC
GATCGCGATGCCGCCAACGCTTTCCAGCACTACGTCAATGAGTCTCTAGCTTTTGCCGTC
AAGCGCGGCGGCTTTATGTACGGCACCGTCACCGAGGAAGGACAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 11-K112306-0022-212-B05C-8409
GenBank Accession LN491181 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112306-0022-212-B05C-8409
TCATTGAATTATCCTGTCCTTCCTCGGTGACGGTGCCGTACATAAAGCCGCCGCGCTTGA
CGGCAAAAGCTAGAGACTCATTGACGTAGTGCTGGAAAGCGTTGGCGGCATCGCGATCGA
AGGAGACGGAGTCGCAGTGAGACGTCTCCTGACGAGTGACGCGCATCTGGCGGGCGATCA
AGTCATCGACGGTCATCTTCCTCCCGAAGGATCCGGCAGGAGTAACGGGAGGAGCTCCAG
GAATGGAGCGCTCGCCATCGTAGGCGAGGTAGAGCATAGAGCCATGGCCGAGGTTGAGAG
AGGAGAGGGGGAGGTTTGGGTCCGTGAGGTCGGTGAAGGCGAGTAGATCAGCGGGAGTTT
TGGCGAGGAGGAGATCACGATTGGTGGATAAGGTCTGCTTATGGAGAGGGATCTGAAGCT
GATCCGCGATCAAGGTTTTGAGCTGGGAAACAGTGATATGAGCTCCCTCCGCTGTGACTC
TCTCCAGTCCATCTCTGCTTCGGATTCTGAGCATCATCATCGTCTTCCTTCTTTCT

Last Updated on 06.12.2018 16:11