Sequences from confirmation amplicon


Confirmation sequences for line 212D04 and hit At5g45970 are shown.
Line ID 212D04
Locus At5g45970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18644437
Primers used for PCR TB01 - 8474, show primer details
   
Sequence Name 19-K020131-022-212-D04C-TB01
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, TB01: 5'-CTTTTGCAAATGAAAAACGTATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K020131-022-212-D04C-TB01
GGGGGGGCCCGGGGATAATGGTTTTTCTCCCGGAGGCCCCCTTTATTCCCCCAATAATTT
ATTCCTCCGGGGGCACCAAATTTTTTTGAAAAACCTTTTTCCATAAACCCAAGTTCTTTT
CTTTTAAAAAAAAACGGGGGGAAAAACTTTTAAAGTACCCCTTTTTGGAAAATTTCCCCA
CCGGGGGGGGAAATTTCAATTTGAAAGGGGTCCTTTCCCGGGAAACTTCCTGGGGCCCCC
AAGAGGATTATGGGGCCTATTGGGGAAAAAAAAAAAGAATTTCCCCCTTTTTTTTCATTC
CAAAAAGGAGAAGGCCCCCCCCCTTTTTTTAAAAGAAAAGACCTTTTTTTTAAACCACCA
AATTCCAACCCCCCCATTTTTTTGGGGGAGCCATTAACCAATTTTTTTTATTTCGGAAAA
TTTTTTTTTACCGGG
   
Sequence Name 20-K020131-022-212-D04C-8409
GenBank Accession LN495023 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K020131-022-212-D04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCAGTTACGAGAATAT
TCACAAAAAGGTAACTTTAAAAGTTTTTCCATCCGTTTTTTTTTATAGATAAGAAACTAT
GATTATATGATAAAGTATTTCTAGAAAAATTTGTTGACACTGACTGATTAATTAATCTGG
TGATTAATAGTGGCTTCCGGAGCTGAAACATTATGCTCCTGGCATCCCCATTGTGCTCGT
CGGAACAAAATTAGGTAACGAAAACTTTCCCCTTATGAGTTCATAAAGAAACTTAATTTA
TCAACATTTGTGTCAAATTAGATATGGTCTTGAGTAATAATCCGCAGATCATACGTTTTT
CATTTGCAA

Last Updated on 06.12.2018 16:11