Sequences from confirmation amplicon


Confirmation sequences for line 213C04 and hit At2g17420 are shown.
Line ID 213C04
Locus At2g17420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7564404
Primers used for PCR 08sf - 8474, show primer details
   
Sequence Name 58-K116453-0022-213-C04C-08sf
GenBank Accession LN509803 [GenBank]
Sequencing primer gene specific primer, 08sf: 5'-TTTGTTTAGGTGTTAGGGATAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116453-0022-213-C04C-08sf
AGTTTATGTTTCCAAATTTTATGGTTTAATTTATACTCTCAGATTAGGTTAAACATTTTT
ATTTTTAAAAATCTATAATATTTTTCTAATTTTACTTTAAATTGTAGACTAAATAATTGA
TTCAGCCTTATATTATATCTTAAACTTTGCAAGAATCACTTTGGCTTGTAATGAAATGAA
ATCCACTGTTTTCATATTCAAAGTGATTCTAGTCTTGTATTAACTATTAAATATTCCAAT
CTTTAACAACAAAGAAAACTATAATATTCAAAGCCATGTATATAATAATGGACATGGTCC
AATAATTTGGCAATTTAAAAAAATATTAAAGAGAAAAGTAGATTCAAATAAAGTGGTCTA
TATTTCAAATTGCAAAATGTGTTGGATCTCAATGAGCCAGTCAAGATTCATTATAAAGTC
TTTATTTAGCACAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 25-K116452-0022-213-C04C-8409
GenBank Accession LN497611 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K116452-0022-213-C04C-8409
ATTTCATTGAATATATCCTGCTGTGCTAAATAAAGACTTTATAATGAATCTTGACTGGCT
CATTGAGATCCAACACATTTTGCAATTTGAAATATAGACCACTTTATTTGAATCTACTTT
TCTCTTTAATATTTTTTTAAATTGCCAAATTATTGGACCATGTCCATTATTATATACATG
GCTTTGAATATTATAGTTTTCTTTGTTGTTAAAGATTGGAATATTTAATAGTTAATACAA
GACTAGAATCACTTTGAATATGAAAACAGTGGATTTCATTTCATTACAAGCCAAAGTGAT
TCTTGCAAAGTTTAAGATATAATATAAGGCTGAATCAATTATTTAGTCTACAATTTAAAG
TAAAATTAGAAAAATATTATAGATTTTTAAAAATAAAAATGTTTAACCTAATCTGAGAGT
ATAAATTAAACCATAAAATTTTGGAAACATTAAACTCTAAATAGTAAACACTAATAAACT
TCGGAAAAAATAAACCCTATCCCTAACCGTTAAACAAAA

Last Updated on 06.12.2018 16:11