Sequences from confirmation amplicon


Confirmation sequences for line 213D06 and hit At4g35460 are shown.
Line ID 213D06
Locus At4g35460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16843410
Primers used for PCR AU10 - 8474, show primer details
   
Sequence Name 13-K106588-0022-213-D06C-8409
GenBank Accession LN492052 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106588-0022-213-D06C-8409
TCATTGAATATATCCTGTGACCCTATGGCTTGTCAAGTTCTTGAGGTTTAGTTGCTTCTA
TATCATGTGATTCAATTCACAATTGATGCAAGCCTAGTTTTTGTTTTGGAGTTGTTCTTC
TTTGAGTTTGTTGTTCCATGCTTAGTAGAGAGATCATTGATTGTTTTAGGTAACAGTCTT
TAGAGTAAAGAGTCTTGTTTTCACAATGTTCAATGCTTATTCTATTCATAGAGCAATAAT
CGTCATTGCAAACTCTGTTTGATTGATTGTGTAGGGTGCATGGCAGCTTTGGATGCAGAG
CATTACTTACAAGAGATTGGATCTCAGCAAGGTAAGAGTGATTGAGGAAATTGTTGGAAG
GCTCAGGTCATTGAGTTATTGTTGATTGATCCCTGATTCAGTTAGACATTGATGGACCAG
AATGAGACTTGATCTTTATCATAGAACAAGTTAAGATACATTGATTTGTTTATTCCTTAA
TATTGCAGTTGGAATTGAGTACGGGTATTG
   
Sequence Name 29-K106589-0022-213-D06C-AU10
GenBank Accession LN499302 [GenBank]
Sequencing primer gene specific primer, AU10: 5'-CAATACCCGTACTCAATTCCAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106589-0022-213-D06C-AU10
AGATACAATCATGTATCTAACTTGTTCATGANAAAGATCAAGTCTCATTCTGGTCCATCA
ATGTCTAACTGAATCAGGGATCAATCAACAATAACTCAATGACCTGAGCCTTCCAACAAT
TTCCTCAATCACTCTTACCTTGCTGAGATCCAATCTCTTGTAAGTAATGCTCTGCATCCA
AAGCTGCCATGCACCCTACACAATCAATCAAACAGAGTTTGCAATGACGATTATTGCTCT
ATGAATAGAATAAGCATTGAACATTGTGAAAACAAGACTCTTTACTCTAAAGACTGTTAC
CTAAAACAATCAATGATCTCTCTACTAAGCATGGAACAACAAACTCAAAGAAGAACAACT
CCAAAACAAAAACTAGGCTTGCATCAATTGTGAATTGAATCACATGATATAGAAGCAACT
AAACCTCAAGAACTTGACAAGCCATAGGGTCACAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA

Last Updated on 06.12.2018 16:11