Sequences from confirmation amplicon


Confirmation sequences for line 214C06 and hit At2g21390 are shown.
Line ID 214C06
Locus At2g21390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9156033
Primers used for PCR MR58 - 8474, show primer details
   
Sequence Name 53-K017281-022-214-C06C-MR58
GenBank Accession LN507985 [GenBank]
Sequencing primer gene specific primer, MR58: 5'-GAAAATTCACCAAAAGAAAATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K017281-022-214-C06C-MR58
ATTACGTCTATATTTCTCAATCGAGTTGAAAATCCGATAAGCCTAAATGAAATGTCCTGA
GACTGCCAAAATAGGATCAAACAAAAAGAGATAAAGCAAGAAGATAAAGTTCTCATGCGT
TTGTTACTAAGATAAGAAACAACATAAAGACTTTAAAGAGTAATAGAAGTTGCAGCGGAA
AATTCACAAACACCAAAAGCTCAACACTCTTCTTCCATAAAAGAGCATCTCGTACTTACC
TCACGATTGAAGCACATAATCATTCTGACTTGTTCACCGGACTTGAGAGGCAGAGCATAG
CAAGCCAGATGCGTCTGCGCCAATGACTGCAAGATCACAGACCGAGCAAATGTTACCTTC
CTGGCTTGGTACAAACCGGGCAGTGCAGTAAGGACATGCAACGTCTTTCTGTCCTTTGTA
GATTGGCACATAAGTGGACCCGCATATCACAAACGGGTTTCTAAAGTCGTAGTTGAGTGT
TGTTGCATCTGTCATGTTGCGTTCAGCTGCTTGCATAACTTGTCTAGCCGTCCTGGCCTG
GCTCTCAATAGTTGGGTTTGTATCCAACAAACTTCTGGCGAAGTGAGCAGCAGTAGCCAT
GTTCTTTGACTTGTAGCAGACGGTCATTGCACTGAAGTACGCGAGCCGTAGGTGAGGTGT
TTGAAGTTTACAGTGAGTGAAGTAAGCAGCGAGCTCTTGCTGCCTCACCGGATCATCTTT
CATCTCTCTCCTCTTGAGCTCCAATTGAAAGACCGAGGACATATTCTTTAACAATGATGA
ACCA
   
Sequence Name 54-K017281-022-214-C06C-8409
GenBank Accession LN508324 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K017281-022-214-C06C-8409
ATGAAGCCATTTACAATTGAGTCAAGAAGAGAAGTAGATGAGGTTAAGGAACTGGTCATC
ATTGTTAAAGAATATGTCCTCGGTCTTCAATTGGAGCTCAAGAGGAGAGAGATGAAAGAT
GATCCGGTGAGGCAGCAAGAGCTCGCTGCTTACTTCACTCACTGTAAACTTCAAACACCT
CACCTACGGCTCGCGTACTTCAGTGCAATGACCGTCTGCTACAAGTCAAAGAACATGGCT
ACTGCTGCTCACTTCGCCAGAAGTTTGTTGGATACAAACCCAACTATTGAGAGCCAGGCC
AGGACGGCTAGACAAGTTATGCAAGCAGCTGAACGCAACATGACAGATGCAACAACACTC
AACTACGACTTTAGAAACCCGTTTGTGATATGCGGGTCCACTTATGTGCCAATCTACAAA
GGACAGAAAGACGTTGCATGTCCTTACTGCACTGCCCGGTTTGTACCAAGCCAGGAAGGT
AACATTTGCTCGGTCTGTGATCTTGCAGTCATTGGCGCAGACGCATCTGGCTTGCTATGC
TCTGCCTCTCAAGTCCGGTGAACAAGTCAGAATGATTATGTGCTTCAATCGTGAGGTAAG
TACGAGATGCTCTTTTATGGAAGAAGAGTGTTGAGCTTTTGGTGTTTGTGAATTTTCCGC
TGCAACTTCTATTACTCTTTAAAGNCTTTATGTTGTTTCTTATCTTAGGAACAAACGCAT
GAGAACTTTATCTTCTTGCTTTATCTCTTTTTGTTTGATTTTATTTTTACAGTC

Last Updated on 06.12.2018 16:11