Genotyping and primer details


Genotyping and primer details for 214C06 - At2g21390
Line ID 214C06
Locus At2g21390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9156033 of line 214C06 Chr2:9156033
   
Insertion allele amplicon 8474 - MR58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR58: 5'-GAAAATTCACCAAAAGAAAATGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9156898
   
wt amplicon (over InsPos) 27dy - MR58
wt amplicon start Chr2:9155660
wt amplicon end Chr2:9156898
wt amplicon size 1239 bp
Locus specific primer MR58: 5'-GAAAATTCACCAAAAGAAAATGGA-3'
2nd locus specific primer 27dy: 5'-ATTTGGAGTCAGAAATCTTCCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11