Sequences from confirmation amplicon


Confirmation sequences for line 214F01 and hit At2g31810 are shown.
Line ID 214F01
Locus At2g31810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13526637
Primers used for PCR 51et - 8474, show primer details
   
Sequence Name 29-K110678-0022-214-F01C-51et
GenBank Accession LN499338 [GenBank]
Sequencing primer gene specific primer, 51et: 5'-GCCATCAAGACATTCAAACTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110678-0022-214-F01C-51et
CTTTTGGTATCCCACCTCATGCACATCTACGAGTTTGTAAAGTTGCTGCACCAATTTGCT
GACCGATTCATCTGTTGCAGGTATAACTGTTGTAATGCGTGAAATGCCCTTGGTTTCAGC
ATGTCCTACGGCCAAGCTCTAAAAGAAAGGAGATCTTTCAGTGTGTCAGTAAACCTAAGC
ACACTGTGTGTGCGTGTATGAGAGAGATAAGGACTATGCCTGGATATTGTATCCCCTTCG
AGCGAAAACACCAGTCACAATATTAAGAACTCCTGGAATATCATTTACAAGCAATGATAG
AGTATGCGACCGTAGTCCACTCGTCTGAAAGATCAAAAAAGAATAACTGTGATAAATAAA
ACGAAATAGCTTAAAGAAAACCCCAACTCTTTCAATGTCTGCTTTGTAAGTAAATACATA
CAGTCTCCAAATCTAACACTCAATTGATTTATCATTTATGTAACTATATNAACAGAATTG
ATTTCAATTGATTTATCANTTATGTAACTATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGGTCATATGGAGAAAAAGAAAGAGTAATT
ACCAA
   
Sequence Name 13-K110677-0022-214-F01C-8409
GenBank Accession LN492090 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110677-0022-214-F01C-8409
CGGAATGAGCCTTTACATTGAATATAGTTACATAAATGATAAATCAATTGAAATCAATTC
TGTTAATATAGTTACATAAATGATAAATCAATTGAGTGTTAGATTTGGAGACTGTATGTA
TTTACTTACAAAGCAGACATTGAAAGAGTTGGGGTTTTCTTTAAGCTATTTCGTTTTATT
TATCACAGTTATTCTTTTTTGATCTTTCAGACGAGTGGACTACGGTCGCATACTCTATCA
TTGCTTGTAAATGATATTCCAGGAGTTCTTAATATTGTGACTGGTGTTTTCGCTCGAAGG
GGATACAATATCCAGGCATAGTCCTTATCTCTCTCATACACGCACACACAGTGTGCTTAG
GTTTACTGACACACTGAAAGATCTCCTTTCTTTTAGAGCTTGGCCGTAGGACATGCTGAA
ACCAAGGGCATTTCACGCATTACAACAGTTATACCTGCAACAGATGAATCGGTCAGCAAA
TTGGTGCAGCAACTTTACAAACTCGTAGATGTGCATGAGGTGGGATTACAAAAGCTACTG
TCTTTCTTATATATNTAACAGTTTGAATGT

Last Updated on 06.12.2018 16:11