Sequences from confirmation amplicon


Confirmation sequences for line 215A11 and hit At3g22000 are shown.
Line ID 215A11
Locus At3g22000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7752304
Primers used for PCR KT64 - 8474, show primer details
   
Sequence Name 76-K100816-0022-215-A11C-8409
GenBank Accession LN513348 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K100816-0022-215-A11C-8409
GCCTTTACAATTGAATATATCCTGAATCAAACATAAATTGTTGTATACAAATAATCACAG
AACATAATATTTCTTATTTAAAACAATTTGTTTTGGCTAAAATAACATTTAATATGATAT
GAAGAGAGTAAATAATGTTACCCCGGAGATGCCGGCAGCATAGCAAGAGCGGCACTTAGA
ATTATAGGAGTCACCGCTGCACTGGTAGAGAATGGTTACAGCATTGGGTTTTAGACCACT
AGTCATGTGCATGGAACCTGAGCGAAAATTTCCGGCTGCGAGAAAACGGATGTAATCGTT
AAGGATATACTCGTACTTGCTTCCTGGTTTATATTTCCCTT
   
Sequence Name 76-K100828-0022-215-A11C-KT64
GenBank Accession LN513350 [GenBank]
Sequencing primer gene specific primer, KT64: 5'-GAAGGGAAATATAAACCAGGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K100828-0022-215-A11C-KT64
TCCGTTTTCTCGCAGCCGGAAATTTTCGCTCAGGTTCCATGCACATGACTAGTGGTCTAA
AACCCAATGCTGTAACCATTCTCTACCAGTGCAGCGGTGACTCCTATAATTCTAAGTGCC
GCTCTTGCTATGCTGCCGGCATCTCCGGGGTAACATTATTTACTCTCTTCATATCATATT
AAATGTTATTTTAGCCAAAACAAATTGTTTTAAATAAGAAATATTATGTTCTGTGATTAT
TTGTATACAACAATTTATGTTTGATTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11