Sequences from confirmation amplicon


Confirmation sequences for line 215C08 and hit At5g65140 are shown.
Line ID 215C08
Locus At5g65140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26020930
Primers used for PCR MR61 - 8474, show primer details
   
Sequence Name 33-K017368-022-215-C08C-MR61
GenBank Accession LN500791 [GenBank]
Sequencing primer gene specific primer, MR61: 5'-GATTATGACGGTACTCTTTCTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K017368-022-215-C08C-MR61
TCATGACTACCAAGGTTCTTATAATCAATCAAGATCGATTTCCATAATTTTCTGTTTTTT
GTTTTGATTTGCAAATCTCTTATTTTTGTATATATCAATTTATGTTTTATGTTTCACAGA
TGAGAAGAACAGTGAAAAAAATGGCTAAGTGTTTTCCAACTTCCATAGTTACTGGTAGAT
GCATCGACAAGGTACATTTTGTTAGACAATTTTATTTCTCATTTCTCTTAACAAAACATG
ATAATGAACCATTTTCAAATAATTATCTTCTGTTAATGAATATTTTTGTAGGTTTATAGC
TTTGTGAAGCTAGCAGAACTGTATTATGCTGGAAGCCATGGGATGGATATTAAAGGCCCA
ACTAAAGGTTTCTCAAGATACAATAAGGTAGACTCTTGAATTCACATGGTTAGAGAATTT
CAATCCTGATTTTCTTTAGTTTTGTATTTTCTCTTAACAAAATGTTACTTTTGGTTTTTA
GGATAAACCATCCGTTCTTTACCAACCAGCCGGCGACTTTCTTCCCATGATCGATGAGGT
AAATAAATTTGGGTTTTTATTTTATTCTTTTTATCATCTCTGTATGTATCTGATTTTAGA
ATTTTTTCGTTTTATTTTCAGGTTTATAAACAATTAGTGGAGAAAAACAAGTTCTGTCTT
TC
   
Sequence Name 37-K017368-022-215-C08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017368-022-215-C08C-8409
TAGATTTCTCGGACTCTGAAGCCATTTACAATTGAATATATCCGGATAGACAGAGGGACG
GAGCGCGCTTATCGACACTACTTGTTTTTCTCCCCGAATTGATTATGAACCTGTTCTTAA
ATCGGATACATTCTGAGATGATATACATACTGAGATGATAACACGAATTTATTTACCTCA
TCGATTTTGGGACCACAGCCGTCGGGGGGATGGAGACGACCGGTGGGTGGATCCTAACGG
CCGGATGTTCCTTTTTGCCAAGAGAAACTTTTTGACTAGAGAAAATCCGGATCGAAATTC
TCTCACGATGGGAATTCTCGAGCCTTGCGTATTGTATAGTGTGACTCCTTGAATCGGGAC
ATTCCTATCCATGGGCCGGTTTTCTCCATCCCACGGCTCTGCTAGTTTCACAGAGCTGTT
AGCTTCCCAAAGTTTTCATCCTCCGAAGATATTCATTTGCAGATGGTTCTTTTTCGTGAA
TGGTTCATAGACATGAGATGTTAAGTGGTCTGACAAATTGTACCGTGTCGATGCATGTAC
CTTGACCTTGGGTCGACGGAAAACTCT

Last Updated on 06.12.2018 16:11