Sequences from confirmation amplicon


Confirmation sequences for line 215D03 and hit At1g77220 are shown.
Line ID 215D03
Locus At1g77220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29013663
Primers used for PCR 02im - 8474, show primer details
   
Sequence Name 77-K112969-0022-215-D03C-02im
GenBank Accession LN513574 [GenBank]
Sequencing primer gene specific primer, 02im: 5'-CCAAGACTCCAATCTTTTACGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K112969-0022-215-D03C-02im
ACCCCATAAGACATGTGCCTTCTAGAAGAGGAGTACTCTGGGTGATAACTGTCTGCTGTT
CCATGAATTCAATTGTACGTTCTTCTCCATCTGCGGATATAGAGAAGAAATAGACTTGTA
AATTATAACTGAGAAATAATCAAAAGTCAACAGATTCCAAATATATATATTTTTAACTGA
ATAAAGTATGGCTTCAATAATCAACCACTTGCTTATTTGAAGCCCAAAAGATCATAGTCA
GTGACTCAGAAGCTATGGATACAGACTTTGACGGTTATATAAGCATTACCTAGGCAAGCT
ATCAAATATCTCTCGAAACAATAGAGTGCAAAAGCTTCATAACAATCTCTAATCACCTCG
CAGTTGAAAGCTGCTTCTGAATTCACCAAAGACAAAAACTGCTCGGAAATTATAAGAAAC
CCCGAATTAGAAAAATATCTGAACACAAATCTTAATGGAAATTTCACTACAAATACAGGA
TTAAAGTATATAGTAACTTATTAAACCTACCGATTCCACGGCATAAACAGCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTA
   
Sequence Name 70-K112968-0022-215-D03C-8409
GenBank Accession LN512418 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K112968-0022-215-D03C-8409
CGGATGAGCATTTCATTGCTGTTATGCCGTGGAATCGGTAGGTTTATAAGTTACTATATA
CTTTAATCCTGTATTTGTAGTGAAATTTCCATTAAGATTTGTGTTCAGATATTTTTCTAA
TTCGGGGTTTCTTATAATTTCCGAGCAGTTTTTGTCTTTGGTGAATTCAGAAGCAGCTTT
CAACTGCGAGGTGATTAGAGATTGTTATGAAGCTTTTGCACTCTATTGTTTCGAGAGATA
TTTGATAGCTTGCCTAGGTAATGCTTATATAACCGTCAAAGTCTGTATCCATAGCTTCTG
AGTCACTGACTATGATCTTTTGGGCTTCAAATAAGCAAGTGGTTGATTATTGAAGCCATA
CTTTATTCAGTTAAAAATATATATATTTGGAATCTGTTGACTTTTGATTATTTCTCAGTT
ATAATTTACAAGTCTATTTCTTCTCTATATCCGCAGATGGAGAAGAACGTACAATTGAAT
TCATGGAACAACAGACAGTTATCACCCAGAGTACTCCTCTTCTAGAAGGCACATGTTCTT
ATGGGGTTGTAGAGCATCCCTTTCCAATGAACTGCTTCGTAAAAGATTGGAGTCTTGG

Last Updated on 06.12.2018 16:11