Sequences from confirmation amplicon


Confirmation sequences for line 215E10 and hit At3g53500 are shown.
Line ID 215E10
Locus At3g53500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19835833
Primers used for PCR KS24 - 8474, show primer details
   
Sequence Name 59-K031452-022-215-E10C-KS24
GenBank Accession LN509897 [GenBank]
Sequencing primer gene specific primer, KS24: 5'-TAGTACCTGGCCTTCTTTGGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K031452-022-215-E10C-KS24
TCTTTCACCCAGCGGTACATTTATTCTTCCAGTCTCCTGCTGTGCAGTCTCGGGCCCAGT
GGCCATCGACACCACAATTAAAACAGCGACCAGAACCAGGAGGTGGGCCTCTGCTACCAT
TGTCCCGCGAACCACGAGGAGCCTATCATAAATTCAAAAGTTTGGCAAGATATCAGTAAT
TTTTCATCCAAATTATCAAGCCAATGGAGAATCGGTAATACAGACACAAATAACTAACCC
CTCTTGATGCCTCCACAGTGATGCGACTTCCATCGAAATCCCGTCCATCCAAGTAATATC
TTGCGTCATCAGCATCACGAGGATCACTAAATTCCTGTTCTCAAAATGTGAGGGACAATG
ATAGATGCATAACATATATATAAAGTTTGTGCGCATGACAAACTTGAAAATCTACATATA
TATAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCACTCCCAAATGG
   
Sequence Name 51-K031452-022-215-E10C-8409
GenBank Accession LN507397 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K031452-022-215-E10C-8409
GCCTTTACAATTGAATTATATATATGTAGATTTTCAAGTTTGTCATGCGCACAAACTTTA
TATATATGTTATGCATCTATCATTGTCCCTCACATTTTGAGAACAGGAATTTAGTGATCC
TCGTGATGCTGATGACGCAAGATATTACTTGGATGGACGGGATTTCGATGGAAGTCGCAT
CACTGTGGAGGCATCAAGAGGGGTTAGTTATTTGTGTCTGTATTACCGATTCTCCATTGG
CTTGATAATTTGGATGAAAAATTACTGATATCTTGCCAAACTTTTGAATTTATGATAGGC
TCCTCGTGGTTCGCGGGACAATGGTAGCAGAGGCCCACCTCCTGGTTCTGGTCGCTGTTT
TAATTGTGGTGTCGATGGCCACTGGGCCCGAGACTGCACAGCAGGAGACTGGAAGAATAA
ATGTTACCGCTGTGGTGAAAGAGGACACATTGAGAGAAACTGCAAAAACAGTCCTAGTCC
AAAGAAGGCCAGGTACT

Last Updated on 06.12.2018 16:11