Sequences from confirmation amplicon


Confirmation sequences for line 215E11 and hit At5g11100 are shown.
Line ID 215E11
Locus At5g11100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3532849
Primers used for PCR IF39 - 8474, show primer details
   
Sequence Name 52-K101385-0022-215-E11C-8409
GenBank Accession LN507759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101385-0022-215-E11C-8409
GCCATTTACAATTGAATAATCCTGGAAATCTATGAAAAGTTACCTCCTTTACATAAGGCC
AGATCTTTTCAAGTTCAAGATTAAGCCAATTTAACTGTAGTTTAATCAAGGACAAGTATC
AATACATCAACAAGAGCAAAAGACAGAACAAAATGATGAAAGTACAAGACATTTAGAATA
ACTCAACTTTTGTCTCTGAGAAAACACAACCCAAGAAGGATAAAAATCTCCCGGGAGAAG
TTTTCTCGAATCTTGAACTGTCATTCTTGCAAATGCAGCAATGGTCTTTGCCTATTCAAA
ACCAAAAAATTGTTCTTCTTATTTATTAATCTTCTTAGATGGATTAATCACAATACGAAA
ATATGAAATTGGTTTTACCAAATCAGCTCGACGAGTGGATCTGACACTAGAGTAGCGAGC
AAATGCGACGACCAAACCGAAAGAAACCGCGATTCCTATGAACAAACCAAAGAGAANACC
CATTTATGTTCACAC
   
Sequence Name 52-K101391-0022-215-E11C-IF39
GenBank Accession LN507760 [GenBank]
Sequencing primer gene specific primer, IF39: 5'-AAGTGTGAACAAAAATGGGTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K101391-0022-215-E11C-IF39
ATCGCGGTTTCTTTCGGTTTGGTCGTCGCATTTGCTCGCTACTCTAGTGTCAGATCCACT
CGTCGAGCTGATTTGGTAAAACCAATTTCATATTTTCGTATTGTGATTAATCCATCTAAG
AAGATTAATAAATAAGAAGAACAATTTTTTGGTTTTGAATAGGCAAAGACCATTGCTGCA
TTTGCAAGAATGACAGTTCAAGATTCGAGAAAACTTCTCCCGGGAGATTTTTATCCTTCT
TGGGTTGTGTTTTCTCAGAGACAAAAGTTGAGTTATTCTAAATGTCTTGTACTTTCATCA
TTTTGTTCTGTCTTTTGCTCTTGTTGATGTATTGATACTTGTCCTTGATTAAACTACAGT
TAAATTGGCTTAATCTTGAACTTGAAAAGATCTGGCCTTATGTAAATGAGGTAACTTTTC
ATAGATNTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTN
CATTCANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11