Sequences from confirmation amplicon


Confirmation sequences for line 215E12 and hit At5g16970 are shown.
Line ID 215E12
Locus At5g16970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5577653
Primers used for PCR 13cp - 8474, show primer details
   
Sequence Name 63-K108745-0022-215-E12C-8409
GenBank Accession LN511006 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K108745-0022-215-E12C-8409
TTTTTTGCTTGTCATCGTGTTGACTGTTGTTGTTGCCGTTGTGTGTTTTGGTCTATTCAA
TCCAAGGGTATGGAGTGTCTAGAATAATAGAATCTGGACATCCAGATTACAAGAAAGGAG
ATTTACTCTGGGGTATAGTTGCATGGGAGGAGTACAGTGTTATCACTCCAATGACTCACG
CGCATTTCAAGATCCAACATACTGATGTTCCATTATCTTATTACACTGGACTTTTAGGTA
TGTTAATCTCAACCTTTTTCATAGTTTTGTAAGGTGGAATCACTTTGTAAAATTTTGGAA
ATCTGCTTCTTGATGATACAATGTTTTGACTTGAGACAGTGAGGTCATAACCTTTATTAT
GGATTTCTAAAAAATCTTAGAAACATATGAATGGAATTGGTTATAACTTCAGTCTAGGGT
TTCAACTGTTTTGTTTGTTGATCTATTTCATATATTCACTCTTTGATGGGTTTTTGTCTT
GTCCTAATAGGTATGCCTGGTATGACTGCCTATGCTGGGTTTTATGAAGTTTGTTCTC
   
Sequence Name 79-K108746-0022-215-E12C-13cp
GenBank Accession LN513838 [GenBank]
Sequencing primer gene specific primer, 13cp: 5'-GGAGAACAAACTTCATAAAACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K108746-0022-215-E12C-13cp
GCATCCTATAGGACAGACAAAAACCCATCAAAGAGTGAATATATGAAATAGATCAACAAC
CAAAACAGTTGAAACCCTAGACTGAAGTTATAACCAATTCCATTCATATGTTTCTAAGAT
TTTTTAGAAATCCATAATAAAGGTTATGACCTCACTGTCTCAAGTCAAAACATTGTATCA
TCAAGAAGCAGATTTCCAAAATTTTACAAAGTGATTCCACCTTACAAAACTATGAAAAAG
GTTGAGATTAACATACCTAAAAGTCCAGTGTAATAAGATAATGGAACATCAGTATGTTGG
ATCTTGAAATGCGCGTGAGTCATTGGAGTGATAACACTGTACTCCTCCCATGCAACTATA
CCCCAGAGTAAATCTCCTTTCTTGTAATCTGGATGTCCAGATTCTATTATTCTAGACACT
CCATACCCTTGGATTGGCTACACCAAAACACACAACGGCAACAACAACAGTCAACACGAT
AGACATAGCANAAAAACAGAACCAAATTCAATTGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATT

Last Updated on 06.12.2018 16:11