Sequences from confirmation amplicon


Confirmation sequences for line 216H03 and hit At5g43285 are shown.
Line ID 216H03
Locus At5g43285 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17370606
Primers used for PCR 14rq - 8474, show primer details
   
Sequence Name 33-K116239-0022-216-H03C-14rq
GenBank Accession LN501137 [GenBank]
Sequencing primer gene specific primer, 14rq: 5'-AGAATAAATCCATGTGTAGGGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116239-0022-216-H03C-14rq
AGTTTAAAACAAAACAAACATACATAGGTTACTTAACTTTCGTTTATAACAAAATAAAAC
GTTTATTTGAAAATTAACTCTTTGTATAACATATGTATCTATAGAGCCTATAAATATGAC
TGTATATTGTTTTAATAACTCCAGCACAAAACAAAAGAAAAAAGAATTCAAAACGATTCA
CAAATTTTTTTTGTAATTTTTTTTTTGTGGTATTGGTTAATTTCTATGAAAAAATGAAGT
TGATTTTTATATTTTTAACTCTATAAATTTTTGTTTCTTCATGTAAGTCTAAATTTATTT
AATTAGGATAATATATCAGTATTATAATCAACAACAAAAAATTGTTTAAAAATTTGATAT
ATCTTTTCAAAATTTGATAGGTACATCAATACTTATAAAGGAATCTAGTGAAGAAAAGAG
AATATATCCTTTCAATCCAGTAGCAAGTCCTTTCGATCCACGTTCACTGAACCAAATACT
TAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCC
   
Sequence Name 09-K116238-0022-216-H03C-8409
GenBank Accession LN490345 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116238-0022-216-H03C-8409
TTTTAGTATTTGGTTCAGTGAACGTGGATCGAAAGGACTTGCTACTGGATTGAAAGGATA
TATTCTCTCTTCTTCACTAGATTCCTTTATAAGTATTGATGTACCTATCAAATTTTGAAA
AGATATATCAAATTCTTAAACAATTTTTTGTTGTTGATTATAATACTGATATATTATCCT
AATTAAATAAATTTAGACTTACATGAAGAAACAAAAATTAATAGAGTTAAAAATATAAAA
ATCAACTTCATTTCTTCATAGAAATTAACCAATACCACAAAAAAAAAATTACAAAAAAAA
TTTGTGAATCGTTTTGAATTCTTTTTTCTTTTGTTTTGTGCTGGAGTTATTAAAACAATA
TACAGTCATATTTATAGGCTCTATAGATACATATGTTATACAAAGAGTTAATTTTCAAAT
AAACGTTTTATTTTGTTATAAACAAAAGTTAAGTAACCTATGTATGTTTGTTTTTGTTTT
TAAACTATAATTGTTTTGTTACATCAGGAGGTTTTCAACCCACCCTACACATGGATTTAT
T

Last Updated on 06.12.2018 16:11