Sequences from confirmation amplicon


Confirmation sequences for line 217D03 and hit At3g03060 are shown.
Line ID 217D03
Locus At3g03060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:692998
Primers used for PCR 47yt - 8474, show primer details
   
Sequence Name 39-K122490-0022-217-D03C-47yt
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 47yt: 5'-AAGAGTTTAAAGCTATGCAAGCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K122490-0022-217-D03C-47yt
TATTTGGATGTCTGTTTATATATTCTTGAACAATTGTGTTAACAACTAATGGCTACCTAA
TAGAAATGAGTTAGCAACTGTGGCATAACGATTATGCTGTTATCTTAGTAAAGTTGAAAA
TAGTGTCTTGTGACAAAATCTGCTTCCAATGGTCACAAGACACTATTTTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K122489-0022-217-D03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K122489-0022-217-D03C-8409
TTGAAATAGTGTCTTGTGACCATTGGAAGCAGATTTTGTCACAAGACACTATTTTCAACT
TTACTAAGATAACAGCATAATTCGTTATGCCACAGTTGCTAACTCATTTCTATTAGGTAG
CCATTAGTTGTTAACACAATTGTTCAAGAATATATAAACAGACATCCAAATATTGGTTAA
GAAACTTAAAGTTAGATGATCTCACAGTTTCAGCTTGGGCTTGCATAGCTTTAAACTCTT

Last Updated on 06.12.2018 16:11