Sequences from confirmation amplicon


Confirmation sequences for line 217H04 and hit At5g16440 are shown.
Line ID 217H04
Locus At5g16440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5372563
Primers used for PCR MJ62 - 8474, show primer details
   
Sequence Name 15-K029909-022-217-H04C-8409
GenBank Accession LN492843 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K029909-022-217-H04C-8409
ATTGGTAATTGCTCATTGGTGTTTTTATTCAACTCCAAGTATGAGTTGCTTCTCCAGGTA
TTTTCTTATGATCACCTTGATTGTGAAGACGACTCTAGGTTTAGAAATCCAGTATCTGAA
TAGTTTTGTTCACCATTATATTCAGATCATCTAGTCTCTATAGTAATGTCTGAAACAAGT
CACACTTGTAGCTTATGGTGGCCAGCCAATGGACTCGGTGTGTAAACTAGATTTAAGAAA
TTTTATAGCCAAGGCTGTGAAAATCACGTAGCTTTATCATAAAAATGGTTGGTTGGGTTT
TGGCGTTGCTTGCTTC
   
Sequence Name 15-K029909-022-217-H04C-MJ62
GenBank Accession LN492844 [GenBank]
Sequencing primer gene specific primer, MJ62: 5'-GTCACATACTCTGAAGCAAGCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K029909-022-217-H04C-MJ62
CACACATTTTATGATAAGCTACGTGATTTTCACAGCCTTGGCTATAAAATTTCTTAAATC
TAGTTTACACACCGAGTCCATTGGCTGGCCACCATAAGCTACAAGTGTGACTTGTTTCAG
ACATTACTATAGAGACTAGATGATCTGAATATAATGGTGAACAAAACTATTCAGATACTG
GATTTCTAAACCTAGAGTCGTCTTCACAATCAAGGTGATCATAAGAAAATACCTGGAGAA
GCAACTCATACTTGGAGTTGAATAAAAACACCAATGAGCAATTACCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11