Genotyping and primer details


Genotyping and primer details for 217H04 - At5g16440
Line ID 217H04
Locus At5g16440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5372563 of line 217H04 Chr5:5372563
   
Insertion allele amplicon 8474 - MJ62
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ62: 5'-GTCACATACTCTGAAGCAAGCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5372872
   
wt amplicon (over InsPos) 53gc - MJ62
wt amplicon start Chr5:5371975
wt amplicon end Chr5:5372872
wt amplicon size 898 bp
Locus specific primer MJ62: 5'-GTCACATACTCTGAAGCAAGCAAC-3'
2nd locus specific primer 53gc: 5'-CAGAGACGACTCATGTTTGAAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11