Sequences from confirmation amplicon


Confirmation sequences for line 217H10 and hit At3g62820 are shown.
Line ID 217H10
Locus At3g62820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23229899
Primers used for PCR 56yq - 8474, show primer details
   
Sequence Name 59-K122490-0022-217-H10C-56yq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 56yq: 5'-TGCTATCAAAGTAATAATTGCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K122490-0022-217-H10C-56yq
TTGTGGCGTTAGAGTAAATAATTTCAAGTTATTATAGTAAAAGGAATTAAAATGGTTTAT
GGAAAGGACTAAAACGAGTTATAGTAGTTAGACAAAATCTTTTTGAGTGTTTAACCAAGA
AAAAAATATCTTTCGAGTGATTTTTATTAGGTTTTTATCACCGACTTCTTCTCACGTTTT
GACTTGATGAGAGAGAGGTTCGTTACTCGTTAGGTGGCATGGGCTTGTGACGGTCGAATA
AAAATGACAATACTTGACTAATAACATTGGCCCAATATAGGACATTAGGCCCATTATTAT
TTCGCCTGTAAATAATTCCATTATAATGAGAGAGATACAGTTATCGTAGTGATCAGCCAT
GAACCACCTTATTAATAATATGATCTTACACTTTCATGGAAAATTAAAGCTTTTGTAAAA
GTTTATGATTTAAGAGATTACAAACCATTAGCCGCTAGCTTGTTGAGGAGTGCGAGAGAA
TTGCTGGACAAGTACATAGACGTCGTGACTCTCTTCCGAATCATCCTGACCGTTGATGAT
GACCTCGTCGATGTCTTTTCAAACCCATCAAGACACGTGTCCTTGTCAGTGAGGAGCTAA
GCCACGTAGCAAGATCACTCATCTGCTGCTGAAACTCGCTGGCTCTGAGTGTCCGGAGGA
CGGCCAAGGACTTGTGGAGACTATCAAGAGAGTCTTGGAGAAGTTCGCGACAATCCGACA
AAGCGATTCGGTCTCGTTTCCCAATCGTTGAAAGCCTCGTTTTGAGAAGGTGTCTAAGGA
CGTCTTTGTTGTCGGTTATGGCAACGGAAACGCCGGCACGTGCCCATTTGCTGGGACTGT
TCTTGGCGACTGATGCAAACGGC
   
Sequence Name 27-K122489-0022-217-H10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K122489-0022-217-H10C-8409
GCCGTTACCGCTACCAAGACCTCTGCGCTAAAACGCTATTGCCGTTTGCATCAGTCGCCA
AGAACAGTCCCAGCAAATGGGCACGTGCCGGCGTTTCCGTTGCCATAACCGACAACAAAG
ACGTCCTTAGACACCTTCTCAAAACGAGGCTTTCAACGATTGGGAAACGAGACCGAATCG
CTTTGTCGGATTGTCGCGAACTTCTCCAAGACTCTCTTGATAGTCTCCACAAGTCCTTGG
CCGTCCTCCGGACACTCAGAGCCAGCGAGTTTCAGCAGCAGATGAGTGATCTTGCTACGT
GGCTTAGCTCCTCACTGACAAGGACACGTGTCTTGATGGGTTTGAAAAGACATCGACGAG
GTCATCATCAACGGTCAGGATGATTCGGAAGAGAGTCACGACGTCTATGTACTTGTCCAG
CAATTCTCTCGCACTCCTCAACAAGCTAGCGGCTAATGGTTTGTAATCTCTTAAATCATA
AACTTTTACAAAAGCTTTAATTTTCCATGAAAGTGTAAGATCATATTATTAATAAGGTGG
TTCATGGCTGATCACTACGATAACTGTATCTCTCTCATTATAATGGAATTATTTACAGGC
GAAATAATAATGGGCCTAATGTCCTATATTGGGCCAATGTTATTAGTCAAGTATTGTCAT
TTTTATTCGACCGTCACAAGCCCATGCCACCTAACGAGTAACGAACCTCTCTCTCATCAA
GTCAAAACGTGAGAAGAAGTCGGTGATAAAAACCTAATAAAAATCACTCGAAAGATATTT
TTTTCTTGGGTAAACACTCAAAAAGATTTTGTCTAACTACTATAACTCGTTTTAGTCCTT
TCC

Last Updated on 06.12.2018 16:11