Sequences from confirmation amplicon


Confirmation sequences for line 218D01 and hit At1g07280 are shown.
Line ID 218D01
Locus At1g07280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2239797
Primers used for PCR KA30 - 8474, show primer details
   
Sequence Name 26-K105138-0022-218-D01C-KA30
GenBank Accession LN497922 [GenBank]
Sequencing primer gene specific primer, KA30: 5'-AAAGCCTATCCGATTGGTTCTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K105138-0022-218-D01C-KA30
CTCTATGGCTCCCTAATTTGCAATAAAATATCTACCATTCCACGGCTACTTCTGACAGTT
AGTATCAACTTGCAAGACTCCAACCTAAACACCAAACCGAATCTAAAACTCCATAATCTA
ACTTAGTATAATAAAAGAACAAAATCAAAGATAACATGATTAGTAGTAAATTATAATATA
ATGGTCAGATGTTAATTACCTGTCGTAGTCATGAATGATAAGGTAGAGAAACTGTGCGTA
ATTAGCAAGAAGGAGAACGTTTCCAGGCTCCTGAGACAATCCCGTTTGGTAAAGAAGCTC
TGTCTTGAAGTAAACTATTGAGATTTTATGTCTTTATGCATAAGCGGCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K105139-0022-218-D01C-8409
GenBank Accession LN490648 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K105139-0022-218-D01C-8409
TTGAAATATCCTGCCGCTTATGCATAAAGACATAAAATCTCAATAGTTTACTTCAAGACA
GAGCTTCTTTCCAAACGGGATTGTCTCAGGAGCCTGGAAACGTTCTCCTTCTTGCTAATT
ACGCACAGTTTCTCTACCTTATCATTCATGACTACGACAGGTAATTAACATCTGACCATT
ATATTATAATTTACTACTAATCATGTTATCTTTGATTTTGTTCTTTTATTATACTAAGTT
AGATTATGGAGTTTTAGATTCGGTTTGGTGTTTAGGTTGGAGTCTTGCAAGTTGATACTA
ACTGTCAGAAGTAGCCGTGGAATGGTAGATATTTTATTGCAAATTAGGGAGCCATAGAGA
TATTTTAAGCTAGAAATTGTCATAATCAAATCACCATAAGAACCAA
Primers used for PCR AL07 - 3144, show primer details
   
Sequence Name 71-K106402-0022-218-D01C-3144
GenBank Accession LN512567 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K106402-0022-218-D01C-3144
TCCACTATCTTCGCAGACCCTTCCTCTAATAAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCCATGTAAATTTCCCGGACAAGTCTTTCTTTTCTTATGCATTTACAA
TAGTTCTCATGTCTATTTGTGTTTTCCGCTTTGTACCGGCGGTGGTATATTGTTCCCGCC
GCCACCACCGACAAAAGATGTTTTCCCGTAAGGAGAAGAAACAGAGAACGTCTTAACCAT
TGAAGAATCGAATTTCTGATTCGTTTCTTGAGTTTCACTTATCTCCGCCGTCGTTTCGAA
GCTGAAAGAGAGGGTTGTCGGGGGACTTACGGCGGCGGCTAAAGCAGAGTTGCTCTCAAT
AGAGTAAACCGTGAAATAGNCGAGAAG
   
Sequence Name 87-K106403-0022-218-D01C-AL07
GenBank Accession LN514767 [GenBank]
Sequencing primer gene specific primer, AL07: 5'-CTTCTCGCTAATTTCACGGTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K106403-0022-218-D01C-AL07
CCGTAAGTCCCCCGACAACCCTCTCTTTCAGCTTCGAAACGACGGCGGAGATAAGTGAAA
CTCAAGAAACGAATCAGAAATTCGATTCTTCAATGGTTAAGACGTTCTCTGTTTCTTCTC
CTTACGGGAAAACATCTTTTGTCGGTGGTGGCGGCGGGAACAATATACCACCGCCGGTAC
AAAGCGGAAAACACAAATAGACATGAGAACTATTGTAAATGCATAAGAAAAGAAAGACTT
GTCCGGGAAATTTACATGGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATATCACATCACTCCA

Last Updated on 06.12.2018 16:11