Sequences from confirmation amplicon


Confirmation sequences for line 218F05 and hit At4g12210 are shown.
Line ID 218F05
Locus At4g12210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7281384
Primers used for PCR 35xw - 8474, show primer details
   
Sequence Name 86-K119624-0022-218-F05C-35xw
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 35xw: 5'-GTCGTCACAGTTACTTACCGGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K119624-0022-218-F05C-35xw
CAGAATCTTTGAGATAGTTCTCTAGGAAGGATAAAAGCAGAGGAATTGAAGAGTTTGAAG
ATGGAAACAGAGTCTTGTTCAATCTGTCTTGAGAATCTAGTCTCCGGTCCAAAACCTAGC
GACCTCACACGCATGACTTGTTCTCATGTCTTCCATATATCTTTTTCCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 62-K119623-0022-218-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K119623-0022-218-F05C-8409
CATTTACATTGAATATATCCTGGAAAAAGATATATGGAAGACATGAGAACAAGTCATGCG
TGTGAGGTCGCTAGGTTTTGGACCGGAGACTAGATTCTCAAGACAGATTGAACAAGACTC
TGTTTCCATCTTCAAACTCTTCAATTCCTCTGCTTTTATCCTTCCTAGTAGAACTATCCT
CAAAGATTTCTTGTTGTTTGACGTTACCGAGACAGTCCGGTAAGTAACTGTGACGA

Last Updated on 06.12.2018 16:11