Sequences from confirmation amplicon


Confirmation sequences for line 218H12 and hit At4g32850 are shown.
Line ID 218H12
Locus At4g32850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15850628
Primers used for PCR 01ty - 8474, show primer details
   
Sequence Name 25-K117118-0022-218-H12C-01ty
GenBank Accession LN497629 [GenBank]
Sequencing primer gene specific primer, 01ty: 5'-CTCCCTCTAATCATATCGTGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117118-0022-218-H12C-01ty
CAATCGTGTTATGATGGTGGGTACTCAAAATTTAGGTGGTTCGTTGCCGCCGCTGAATTC
TCCTAAAAGCTATGGAATTACGAAGCCGCTCTCTCTTGCTGGGCCTTCTTCGGCGGATAT
TAAGCGTAATGTGGAGCTGGAGAAGGTAAAGCTTTGTTTTTTTGTTTATACAAAAATTGT
TCCACTGTTTAAAGGATTCGTTGATTTGGCTTATCTGATGTGTATTTTCTTATATGCTCA
AGTACTTGGTTGATGAGGGACTCTACGAGAGCAAGGATGATACCATGCGGCGAGAGGAAG
TTCTAGGACGCATCGATCAGGTGACTCAGCGTGTCTCGTTGATTGTTTGTTCTGTATATT
TACAAGTGTTATCTTTTGATGGTTGTGCTAATTGGGTGTCTGAATCGACAAGAATAAAAT
TATTGTGTTTATAATGGTCTTTTAGGTTTCATTGTATTGGTTCTTACTCGTGTATGGTTG
TTGATTTTGGGCTAAGCATAGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 03-K117117-0022-218-H12C-8409
GenBank Accession LN487653 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117117-0022-218-H12C-8409
ATTTCATTGAATATATCCTGATCTATGCTTAGCCCAAAATCAACAACCATACACGAGTAA
GAACCAATACAATGAAACCTAAAAGACCATTATAAACACAATAATTTTATTCTTGTCGAT
TCAGACACCCAATTAGCACAACCATCAAAAGATAACACTTGTAAATATACAGAACAAACA
ATCAACGAGACACGCTGAGTCACCTGATCGATGCGTCCTAGAACTTCCTCTCGCCGCATG
GTATCATCCTTGCTCTCGTAGAGTCCCTCATCAACCAAGTACTTGAGCATATAAGAAAAT
ACACATCAGATAAGCCAAATCAACGAATCCTTTAAACAGTGGAACAATTTTTGTATAAAC
AAAAAAACAAAGCTTTACCTTCTCCAGCTCCACATTACGCTTAATATCCGCCGAAGAAGG
CCCAGCAAGAGAGAGCGGCTTCGTAATTCCATAGCTTTTAGGAGAATTCAGCGGCGGCAA
CGAACCACCTAAATTTTGAGTACCCACCATCATAACACGATTGATAATCCTAAGCGATCA
AAGCAGAAGCGCAGCACAAAAACATAATCTCGCTTCACGATATGATAAGGAGGGAG

Last Updated on 06.12.2018 16:11