Sequences from confirmation amplicon


Confirmation sequences for line 218H12 and hit At5g40280 are shown.
Line ID 218H12
Locus At5g40280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16103696
Primers used for PCR MR66 - 8474, show primer details
   
Sequence Name 57-K017282-022-218-H12C-MR66
GenBank Accession LN509261 [GenBank]
Sequencing primer gene specific primer, MR66: 5'-CTTTATGCATTCGTATCGCTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K017282-022-218-H12C-MR66
GTTTTTTTAATTCTTGCTTAATTCTACTTACTCACTGATCGTTAGGATGCATGATATGGG
AGAAATGGATGTTCGTGCATGCTACACTGCAATTTCGGTGAGTTTTACCAACTTCTATTT
TCCTTTTCTCTGTTTTTGTGGACACCAAAACTTTTTAGGATTAATGAGATCAACAAAGTC
TGGACCCATTATGCTATGTTTCTTCCGTTTTCATGGCTTAAACATCACATTCAGATTACG
ATATGATCTTATTATTTGCACACTTGCGCCCACCAGATACTTTGAATAGAGATTACTCGT
TTTGAGACTTACACGTGTTGCAAATGCATCCTATGGCTGGTTTTCTCCCTGATATGTTTG
ACTTCTCTCTTGTGACACAGGTTGCAAGCATCCTAAATATTATGGATG
   
Sequence Name 58-K017282-022-218-H12C-8409
GenBank Accession LN509568 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K017282-022-218-H12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCAATAACCATCAATAACCAT
GTCATCAAGGAAGAGGGAAGCAAAGCACAATTTTTATCTAAGCCTCAACATAATGGAAGT
AAGTAGGAGACCATACCCACCGTGAGCTTCGGAGCCAGGTTCCCCTCCAATGCCACCTTC
ATAAGTTTGGCAACTGCTCAATATAAATCGAGTTAAAAGAGCTTTCCCAGGGAAATTAAT
GAATTCCTGAATTTACAACTGTATAACAGTAGTTCAGCTATATATATATAATGCGAGATA
AAAGTAATAAGAAAAGCTACCTCAAGATGTAATCTCCTAGGCCCTGGGTGAGTTCATCAT
CCATAATATTTAGGATGCTTGCAACCTGTGTCACAAGAGAGAAGTCAAACATATCAGGGA
GAAAACCAGCCATAGGATGCATTTGCAACACGTGTAAGTCTCAAAACGAGTAATCTCTAT
TCAAAGTATCTGGTGGGCGCAAGTGTGCAAATAATAAGATCATATCGTAATCTGAATGTG
ATGTTTAAGCCATGAAAACGGAAGAAACATAGCATAATGGGTCCAGACTTTGTTGATCTC
ATTAATCCTAAAAAGTTTTGGTGTCCACAAAAACAGAGAAAAGGAAAATAGAAGTTGGTA
AAACTCACCGAAATTGCAGTGTAGCATGCACGAACATCCATTTCTCCCATATCATGCATC
CTAACGATCAGTGAGTAAGTAGAATTAAGCAAGAATTAAAAAAACAACATGACTGAAGGT
ATGGCATTAACAGC

Last Updated on 06.12.2018 16:11