Sequences from confirmation amplicon


Confirmation sequences for line 219C07 and hit At5g20740 are shown.
Line ID 219C07
Locus At5g20740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7026729
Primers used for PCR ZZ72 - 8474, show primer details
   
Sequence Name 61-K021739-022-219-C07C-8409
GenBank Accession LN510402 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K021739-022-219-C07C-8409
AGCCATTTACATTGCGAATCCAGCGAAAGAATAAGACTGTGCAATGCATAGATTAATAAC
AAAAATGTAAATAATTTGTATATGTAAGTAACGATACGAAAAGGAACAAAACTTGTAGAA
TTATTGGCGGACTGGTCACCAGTGCCCAATTATTTTCAAAGAGGTTTCAGAAGTTTGGGT
CCACGTTGGGTTATGAATTGACAAAAGAGGTTTCAGAGACAAAATCTTTATCTGCCATTA
AATGGCTGCCATTTCTTTTGCTCCACTCTTGTAACTTTTAAGGGTAAACTGAATTCTACA
TTACATTTTTACATGCTATTAAAAACGAGTAAAAATTAAATCAAAATTCATATTCTGATA
TTGAATTTGAAAACAAACAGCAAATTTGATTAACCAAGTATAATTTACTAAATAAAATAA
CACCACACTATTTTATCCCAAAGAAATAAAAAATAACACCAAACTATCCTAAAGTTTTGG
TCTGTAATAACTTTAATGATGCTT
   
Sequence Name 62-K021739-022-219-C07C-ZZ72
GenBank Accession LN510644 [GenBank]
Sequencing primer gene specific primer, ZZ72: 5'-GGTCCTTCTAGGAAAGCATCATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K021739-022-219-C07C-ZZ72
ACAACTTTGGATAGTTGGTGTTATTTTTTATTTCTTTGGGATAAATAGTGTGGTGTTATT
TTATTTAGTAAATTATACTTGGTTAATCAAATTTGCTGTTTGTTTTCAAATTCAATATCA
GAATATGAATTTTGATTTAATTTTTACTCGTTTTTAATAGCATGTAAAAATGTAATGTAG
AATTCAGTTTACCCTTAAAAGTTACAAGAGTGGAGCAAAAGAAATGGCAGCCATTTAATG
GCAGATAAAGATTTTGTCTCTGAAACCTCTTTTGTCAATTCATAACCCAACGTGGACCCA
AACTTCTGAAACCTCTTTGAAAATAATTGGGCACTGGTGACCAGTCCGCCAATAATTCTA
CAAGTTTTGTTCCTTTTCGTATCGTTACTTACATATACAAATTATTTACATTTTTGTTAT
TAATCTATGCATTGCACAGTCTTATTTCTTTCGCTGGATTCGCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11