Sequences from confirmation amplicon


Confirmation sequences for line 219F05 and hit At5g23370 are shown.
Line ID 219F05
Locus At5g23370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7864110
Primers used for PCR 59xc - 8474, show primer details
   
Sequence Name 39-K119110-0022-219-F05C-59xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 59xc: 5'-TGTTTTATTATTTGGAACATTTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K119110-0022-219-F05C-59xc
TTTGGTAGTTCAAAAAACTTTAGTTATGTTCTCCTTTATCTATATGCATGTCTAAATGGG
TTTTGTTTCCCTTAAAAAAAGAAGGCAAACAGAGTATATGCCTCCAAAGAAAGTAGCTTT
ATCTATGTGACTTGATGACCCATACATGACTCATCACTTTACCACAAAGGTTCTTAAGTC
TCAACGATTAAGTATTTTGTTTCAACTGCCTCTTGTCTCTTTGTCTTTCCCTTTAAATAC
CTTCCCACTTACACGGTTAATCATCAACCATCACTCACAATCTCTCTCTCTTACTCTTTC
ACAAAATCAAGAATCAAGATCACTACTCAATTCTCCAAGATGACATTGAGCAGAGTTCAC
CAACAACTCATTACATTTCCTGCCGTCAAGACTTCTCCGGCGGGTTACTTGCCTGATCCA
GCTTCCATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATATCCGCAGCGTTATT
   
Sequence Name 16-K119109-0022-219-F05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119109-0022-219-F05C-8409
CATTTACATTGATGGAAGCTGGATCAGGCAAGTAACCCGCCGGAGAAGTCTTGACGGCAG
GAAATGTAATGAGTTGTTGGTGAACTCTGCTCAATGTCATCTTGGAGAATTGAGTAGTGA
TCTTGATTCTTGATTTTGTGAAAGAGTAAGAGAGAGAGATTGTGAGTGATGGTTGATGAT
TAACCGTGTAAGTGGGAAGGTATTTAAAGGGAAAGACAAAGAGACAAGAGGCAGTTGAAA
CAAAATACTTAATCGTTGAGACTTAAGAACCTTTGTGGTAAAGTGATGAGTCATGTATGG
GTCATCAAGTCACATAGATAAAGCTACTTTCTTTGGAGGCATATACTCTGTTTGCCTTCT
TTTTTTAAGGGAAACAAAACCATTTAGACATGCATATAGATAAAGGAGAACATAACTAAA
GTTTTTTGTAACTACCAAATCCGTGTTATGACACAATTTTGTCTAAAGTAACTTCAGTAA
CTACCGAAATGTTCCAAAANATAAAAC

Last Updated on 06.12.2018 16:11