Sequences from confirmation amplicon


Confirmation sequences for line 219F08 and hit At5g22700 are shown.
Line ID 219F08
Locus At5g22700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7546160
Primers used for PCR 15sj - 8474, show primer details
   
Sequence Name 59-K116453-0022-219-F08C-15sj
GenBank Accession LN510083 [GenBank]
Sequencing primer gene specific primer, 15sj: 5'-GCGCTCTAGGTCACTTAACAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K116453-0022-219-F08C-15sj
TGATGATATCGACGAGTGGAAGGTTATCATTGATGCTCCTCGGCTGGCGTATCTGAGTCT
TAAAGATGATCAGTCGGCGAGTTTTGTGATAAGCAATTTGGGTTCATCCGCAAAAGCAGA
TATCAAGGTTAGTTTTAATGTGAACGATATTTGGGATCTACCTGTCACGTTCGAGAGAAG
CAATGTTGGTAAATTACTCACCGGGCTCTCAAGCATAAGGGACCTAACTATAAGTGGAAC
AACTTTGATGGTATACACAGATATATAGTTGAACTTTTGACACTGTCTCTTTGGATAAAA
ACTGCATTTTCTTTGTGTTTTGCAGATCATTTGTCACTACTTGAAGCACGAACCGATGCC
TCAGTTTTGTAACATGGTGCGGTTAAATGCGAGATTCTATGATTGTGATTTGGAAATGTT
GCCATGCGTTCTTGAGAGCTGTCCAAATCTCAAATCCCTTGTCTTGGTAAGTGATTTTTC
TTTATAAAACATGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGGTT
   
Sequence Name 26-K116452-0022-219-F08C-8409
GenBank Accession LN498084 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116452-0022-219-F08C-8409
GAACATGTTTTATAAAGAAAAATCACTTACCAAGACAAGGGATTTGAGATTTGGACAGCT
CTCAAGAACGCATGGCAACATTTCCAAATCACAATCATAGAATCTCGCATTTAACCGCAC
CATGTTACAAAACTGAGGCATCGGTTCGTGCTTCAAGTAGTGACAAATGATCTGCAAAAC
ACAAAGAAAATGCAGTTTTTATCCAAAGAGACAGTGTCAAAAGTTCAACTATATATCTGT
GTATACCATCAAAGTTGTTCCACTTATAGTTAGGTCCCTTATGCTTGAGAGCCCGGTGAG
TAATTTACCAACATTGCTTCTCTCGAACGTGACAGGTAGATCCCAAATATCGTTCACATT
AAAACTAACCTTGATATCTGCTTTTGCGGATGAACCCAAATTGCTTATCACAAAACTCGC
CGACTGATCATCTTTAAGACTCAGATACGCCAGCCGAGGAGCATCAATGATAACCTTCCA
CTCGTCGATATCATCAATAAACCAACCGTTGGAACTATCAAGAACGAGTTTGAGACTGTT
AAGTGACTTTAGAGCGCA

Last Updated on 06.12.2018 16:11