Sequences from confirmation amplicon


Confirmation sequences for line 219F11 and hit At3g02940 are shown.
Line ID 219F11
Locus At3g02940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:662162
Primers used for PCR 13qa - 8474, show primer details
   
Sequence Name 32-K115481-0022-219-F11C-13qa
GenBank Accession LN500710 [GenBank]
Sequencing primer gene specific primer, 13qa: 5'-ACCAGCTATAGACGACCACCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115481-0022-219-F11C-13qa
ATATAACATTGTCAAGCTCTAATAAGATAACTAATTATAGACTTGGTAGTATCGAACATT
GACGAGTACGTATGCATGTACCAAGAAAACTCCAGTGTAGCACGTTATTCATATAAATGC
GTCTATACCTCAACTAACAAAAAAATAAATTATAATAGCATATATAAAAATATATACTTT
TAGACTATATATATGTGATCTATAAATAAGACCGACTGAAAAAGAGACTAAGATATTAGA
AATTTTAAATTTTAATCTGTATTTAATAAAAGAAATGTTTCTTTTTTGCCTAGAACCTAT
CTAATCAAACATTACCATCCTGTAGTTAAATTTTCTTCTTCTTGAATTTTTTTATCAAAA
TGAAATTCTAAAGTGTTAGCAAAAAAGTTCTATTAAAATCACTAATAATAAAAAGCAGTA
AAAAATTCTATAGCACAATAATATTGATTAATTAATAGAAAATATTGATGTGTTTTCTGA
TTTTTGCTCTTGATGATAATCTCACATATATATAATTTATAATTAATCAAAGTAAGTAAG
GGAATTTAAGAAGAAATAAATTAAGATTGATTACTTGTTCCCAAGAAGAGAATGAAGGTT
AATGATAGTTTGTTCTTCCTCAGCTGTGAAGTTTCCTCTTTTGATATCTGGTCTCAAGTA
ATTAGTCCATCTCAATCTGCAACTCTTCCCACACCGGTTTAAACCGGCTTGCTTGGGTAG
AGCTCTCCAGCTTCCATGGCCGTGTTTTCTTATGTGATTTATCAGTTTCTGATCTTCTTC
TGGCGTCCATGGCCCTTTCTTTAATCCGCTCTCATCGCAACACGGTGATCTCCCCATTTT
AAATCTACATGTCCCGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAAGAAAGAGTAATTACCAATTTTTTTTTCAATTTCAAAATGT
   
Sequence Name 04-K115480-0022-219-F11C-8409
GenBank Accession LN488045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115480-0022-219-F11C-8409
GATGAGAGCGGAATTAAGAAAGGGCCATGGACGCCAGAAGAAGATCAGAAACTGATAAAT
CACATAAGAAAACACGGCCATGGAAGCTGGAGAGCTCTACCCAAGCAAGCCGGTTTAAAC
CGGTGTGGGAAGAGTTGCAGATTGAGATGGACTAATTACTTGAGACCAGATATCAAAAGA
GGAAACTTCACAGCTGAGGAAGAACAAACTATCATTAACCTTCATTCTCTTCTTGGGAAC
AAGTAATCAATCTTAATTTATTTCTTCTTAAATTCCCTTACTTACTTTGATTAATTATAA
ATTATATATATGTGAGATTATCATCAAGAGCAAAAATCAGAAAACACATCAATATTTTCT
ATTAATTAATCAATATTATTGTGCTATAGAATTTTTTACTGCTTTTTATTATTAGTGATT
TTAATAGAACTTTTTTGCTAACACTTTAGAATTTCATTTTGATAAAAAAATTCAAGAAGA
AGAAAATTTAACTACAGGATGGTAATGTTTGATTAGATAGGTTCTAGGCAAAAAAGAAAC
ATTTCTTTTATTAAATACAGATTAAAATTTAAAATTTCTAATATCTTAGTCTCTTTTTCA
GTCGGTCTTATTTATAGATCACATATATATAGTCTAAAAGTATATATTTTTATATATGCT
ATTATAATTTATTTTTTTGTTAGTTGAGGTATAGACGCATTTATATGAATAACCTGCTAC
ACTGGAGTTTTCTTGGTACATGCATACGTACTCGTCAATGTTCGATACTACCAAGTCTAT
AATTAGTTATCTTATTAGAGCTTGACAATGTTATATAATAGTATAAAGCTTAACTAACCC
ACCAAAAAACATATTTTTGTCCATATTTTCTAAAGGTGG

Last Updated on 06.12.2018 16:11