Sequences from confirmation amplicon


Confirmation sequences for line 220D09 and hit At5g13600 are shown.
Line ID 220D09
Locus At5g13600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4382489
Primers used for PCR PH26 - 8474, show primer details
   
Sequence Name 24-K017544-022-220-D09C-PH26
GenBank Accession LN496796 [GenBank]
Sequencing primer gene specific primer, PH26: 5'-GGTCTTAGGCCCAATAACTAGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017544-022-220-D09C-PH26
AAATTTTTGATATATAGTTGTGAGATGAACAAGATTTTTAGAAAATTCTTGGATGCATAT
TGAAAGATTTGAATGGTTACAGTGACCACTTTCTATTCACTAGAAGACCAATTTTTTCTA
TCATACCTAAAAAATAGTTTTACTGTCCGCATGTAGTCTTTCAAACCCTATAAGCAAGCA
AGAATTGCATAAAAGAAAAAGGCAAAACATGTAAAATTCAAGAACAAAAGTGATCAAAGT
GTCTTAAGACCATATCATATAACTGTCGTTATTAGTCATTTCTCTTCTCCATCAATTTTT
TAATCATATCTATATTCAATTGTAAAT
   
Sequence Name 28-K017544-022-220-D09C-8409
GenBank Accession LN498638 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K017544-022-220-D09C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGATATGACTAAAAAATT
GATGGAGAAGAGAAATGACTAATAACGACAGTTATATGATATGGTCTTAAGACACTTTGA
TCACTTTTGTTCTTGAATTTTACATGTTTTGCCTTTTTCTTTTATGCAATTCTTGCTTGC
TTATAGGGTTTGAAAGACTACATGCGGACAGTAAAACTATTTTTTAGGTATGATAGAAAA
AATTGGTCTTCTAGTGAATAGAAAGTGGTCACTGTAACCATTCAAATCTTTCAATATGCA
TCCAAGAATTTTCTAAAAATCTTGTTCATCTCACAACTATATATCAAAAATTTACTTTGT
CCTAGTTATTGGGCCTAAGA

Last Updated on 06.12.2018 16:11