Sequences from confirmation amplicon


Confirmation sequences for line 220F11 and hit At2g33220 are shown.
Line ID 220F11
Locus At2g33220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14079729
Primers used for PCR GG12 - 8474, show primer details
   
Sequence Name 47-K100852-0022-220-F11C-8409
GenBank Accession LN506135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K100852-0022-220-F11C-8409
ATTTACATTGAATATATCCTGGATACCCTGACTTCAACATAACTTCTTTTGTGAATCCTA
CCTTTCATCTTCTTCAGCTTGAAGAATCGGTAGAATTGCTCTACGTGCAGCGTACTTCTC
TTCCTTCAACGCCCTATCATCACAGCAATCTTCATTAAAACTTTGGAGTTGATACATTGT
GCTCATTTTGACACTTTTGTCATCATTATGCAATTGAGAGTCTAACAATAGAAGACTATT
AGAGAATGTCACTTACACACACCAAGAAAGATAACACTGTGTTGTCTTATTCGTTGCAAA
TTACTCCAAATCGACACTAATCAAGCTAAATAACTCACAATGTGACCATCTAAAAACCAA
TATGCAACTAACCACTAGTATATCATCTTGCAATTTGATTGCTTTCTTCATCACAGCTCA
AGAGATCGTTAATTAGTAACCAATTTCGAGACTAACCACACAAATTTATAAGCATCAATC
CCAAATTGTCTTAAGATTCAATCCCAAATTACTCAAAATTTCACAATAGAGAAAAGCCTA
ACCAA
   
Sequence Name 47-K100858-0022-220-F11C-GG12
GenBank Accession LN506136 [GenBank]
Sequencing primer gene specific primer, GG12: 5'-ATGTGGAATTTGAGTTTGGTTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K100858-0022-220-F11C-GG12
ATTTGGGATTGAATCTAAGACAATTTGGGATTGATGCTTATAAATTTGTGTGGTTAGTCT
CGAAATTGGTTACTAATTAACGATCTCTTGAGCTGTGATGAAGAAAGCAATCAAATTGCA
AGATGATATACTAGTGGTTAGTTGCATATTGGTTTTTAGATGGTCACATTGTGAGTTATT
TAGCTTGATTAGTGTCGATTTGGAGTAATTTGCAACGAATAAGACAACACAGTGTTATCT
TTCTTGGTGTGTGTAAGTGACATTCTCTAATAGTCTTCTATTGTTAGACTCTCAATTGCA
TAATGATGACAAAAGTGTCAAAATGAGCACAATGTATCAACTCCAAAGTTTTAATGAAGA
TTGCTGTGATGATAGGGCGTTGAAGGAAGAGAAGTACGCTGCACGTAGAGCAATTCTACC
GATTCTTCAAGCTGAAGAAGATGAAAGGTAGGATTCACAAAAGAAGTTATGTTTGAAGTC
AGGGTATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11