Sequences from confirmation amplicon


Confirmation sequences for line 221E10 and hit At2g37975 are shown.
Line ID 221E10
Locus At2g37975 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15891927
Primers used for PCR GD22 - 8474, show primer details
   
Sequence Name 47-K100820-0022-221-E10C-8409
GenBank Accession LN506132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K100820-0022-221-E10C-8409
TTATAACATTTATAAGCCATTGTCTTTAAGCTTTTCTCTGGTTAAAACGATCAAACCCCN
GTTTTTGAATAAACTCAATCCAAACATTTCTGCAACTTCTTTAGCAATTCATTTTATAGT
ATTTAACTTCAAAAGCTCAGATTTGTATACATTATTGTTATGCTTCTTACAACTACAAAC
ACAGTGAAACAAAATAAAAACAAGTTCTTTGAAACCAAAAGCAGGAAACTTTGAATCAAA
TTCATTCAACAGGAATCATGACCTCATTGGTCCTAAACGGTGGTAACGTCCATGGAGGAT
TATACCTAGCAAGAACGAAATCTCCGGTGATCTTAAACCCATCTTTCTCAAGATGACTAC
TCAGCTTCTTCACCTTCTCACTCACCACACTCTCCGACGCTATACCACTAAACTTAATCA
CACCATACTTCCTCCCTCCTTCCTCCTTAATCACAACCCTCTCATCCGTTGGACGTGGTG
CCTCCTCCGCCTTCTTATACATCGACGGCNACAAAAACTGCATCGTCACCAACTTCTTCC
TTCCTTCTCCACCACCTTCCTTAGTTACCACCGGAGAAGTCATCTCAATCTTCTCACTCT
CCTTAG
   
Sequence Name 47-K100832-0022-221-E10C-GD22
GenBank Accession LN506134 [GenBank]
Sequencing primer gene specific primer, GD22: 5'-CTCCGGTGATTACTAAGGAGAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K100832-0022-221-E10C-GD22
GGAGGTGGTGGAGAAGGAAGGAAGAAGTTGGTGACGATCCAGTTTTTGTTGCCGTCGATG
TATAAGAAGGCGGAGGAGGCACCACGTCCAACGGATGAGAGGGTTGTGATTAAGGAGGAA
GGAGGGAGGAAGTATGGTGTGATTAAGTTTAGTGGTATAGCGTCGGAGAGTGTGGTGAGT
GAGAAGGTGAAGAAGCTGAGTAGTCATCTTGAGAAAGATGGGTTTAAGATCACCGGAGAT
TTCGTTCTTGCTAGGTATAATCCTCCATGGACGTTACCACCGTTTAGGACCAATGAGGTC
ATGATTCCTGTTGAATGAATTTGATTCAAAGTTTCCTGCTTTTGGTTTCAAAGAACTTGT
TTTTATTTTGTTTCACTGTGTTTGTAGTTGTAAGAAGCATAACAATAATGTATACAAATC
TGAGCTTTTGAAGTTAAATACTATAAAATGAATTGCTAAAGAAGTTGCAGAAATGTTTGG
ATTGAGTTTATTCAAAAACGGGGTTTGATCATTTTAACCAGAGAAAAGCTTAAAGACCAT
GGCTTAATAAATGTTTATTAATTGTTCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCA

Last Updated on 06.12.2018 16:11