Sequences from confirmation amplicon


Confirmation sequences for line 221G07 and hit At2g32390 are shown.
Line ID 221G07
Locus At2g32390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13749482
Primers used for PCR 06hr - 8474, show primer details
   
Sequence Name 41-K112562-0022-221-G07C-06hr
GenBank Accession LN504134 [GenBank]
Sequencing primer gene specific primer, 06hr: 5'-GTCCTTCTTGAAGCCATTCACTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K112562-0022-221-G07C-06hr
TTTTTCCTTGTTGGACCGTCATCTGGATTCTTGAAATCGATTTAACGAAGAATTCCGCGG
ACCTCCTAGGCGTCAAATCATTACAGTCTTCTGGTTAGTTCCATACAATTATTGAATCGA
CACAAAACTCATATGTATTAGTTTAAGCTCAAGTGCTAAAGCTTTGCTTTACCCCCTTCC
TTTCTCGTCTTCAATATTGCAGGTTTAGCTTCTCAACAATGTTCTTCTCTCACAGTATGC
ACACAATTACATTTAAGATCATACGAAAACATCAAAGAGAAGAAACTCTCGGTTCTTTTC
TTTTGTTACCATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGGTTATTA
   
Sequence Name 17-K112561-0022-221-G07C-8409
GenBank Accession LN493918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112561-0022-221-G07C-8409
TACAAAGAAAGAACCGAGAGTTTCTTCTCTTTGATGTTTTCGTATGATCTTAAATGTAAT
TGTGTGCATACTGTGAGAGAAGAACATTGTTGAGAAGCTAAACCTGCAATATTGAAGACG
AGAAAGGAAGGGGGTAAAGCAAAGCTTTAGCACTTGAGCTTAAACTAATACATATGAGTT
TTGTGTCGATTCAATAATTGTATGGAACTAACCAGAAGACTGTAATGATTTGACGCCTAG
GAGGTCCGCGGAATTCTTCGTTAAATCGATGTTCAAGAATCCAGATGACGGCTCCAACAA
AGAGAAAAAGGGCACCGGTCACAGCCCACATCTCTATAGTGAATGGCTCAAAGAAGGA

Last Updated on 06.12.2018 16:11