Sequences from confirmation amplicon


Confirmation sequences for line 222D11 and hit At2g02470 are shown.
Line ID 222D11
Locus At2g02470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:654609
Primers used for PCR 06do - 8474, show primer details
   
Sequence Name 29-K110307-0022-222-D11C-06do
GenBank Accession LN499335 [GenBank]
Sequencing primer gene specific primer, 06do: 5'-TCTTCTCTGGCATTAGGTTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110307-0022-222-D11C-06do
CTTTAGCTATGCCATGGACCGATTCTTTTTCAGGAAGAGGCTGTTCCAGATGATTAATGA
GCTGCCAACCATTTTCGAAGTTGTGAGCGGCAATGCAAAGCAGTCCAAGGATCTATCTGT
TAACAACAATAATAGCAAAAGCAAACCTAGTGGCGTCAAGGTATCTCTTCTGTTTCACCA
TTTTGTTTTATCAATCTCTGCTAAGTTTTATCTCTTTGTATTCCTCACAAACAGTCTCGC
CAATCCGAATCTCTCTCAAAGGTTGCAAAGATGTCATCTCCACCGCCAAAAGAAGAAGAA
GAAGAAGAAGATGAGAGTGAAGATGAGAGTGAAGACGATGAACAAGGTGCGGTTTGTGGT
GCTTGTGGAGACAACTACGGTACAGATGAGTTCTGGATTTGCTGCGACGCGTGTGAGAAA
TGGTTCCATGGAAAATGTGTGAAGATAACACCTGCAAAAGCAGAACACATCAAGCATTAC
AAATGCCCAACTTGCAGTAACAAGAGAAAAAGAAAGAGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 13-K110306-0022-222-D11C-8409
GenBank Accession LN492087 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110306-0022-222-D11C-8409
GAGCCATTACTCTTTCTTTTTCTCTTGTACTGCAAGTTGGGCATTTGTAATGCTTGATGT
GTTCTGCTTTTGCAGGTGTTATCTTCACACATTTTCCATGGAACCATTTCTCACACGCGT
CGCAGCAAATCCAGAACTCATCTGTACCGTAGTTGTCTCCACAAGCACCACAAACCGCAC
CTTGTTCATCGTCTTCACTCTCATCTTCACTCTCATCTTCTTCTTCTTCTTCTTCTTTTG
GCGGTGGAGATGACATCTTTGCAACCTTTGAGAGAGATTCGGATTGGCGAGACTGTTTGT
GAGGAATACAAAGAGATAAAACTTAGCAGAGATTGATAAAACAAAATGGTGAAACAGAAG
AGATACCTTGACGCCACTAGGTTTGCTTTTGCTATTATTGTTGTTAACAGATAGATCCTT
GGACTGCTTTGCATTGCCGCTCACAACTTCGAAAATGGTTGGCAGCTCATTAATCATCTG
GAACAGCCTCTTCCTGAAAAAGAATCGGTCCATGGCATAAGCTTAAAGGCAAGTGATAGC
TAAGTTGTTCAAACAAACCTAATGCCAGAGAAGA
Primers used for PCR 09eb - 8474, show primer details
   
Sequence Name 29-K110359-0022-222-D11C-09eb
GenBank Accession LN499336 [GenBank]
Sequencing primer gene specific primer, 09eb: 5'-ATCAAATTCTTCTCTGGCATTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110359-0022-222-D11C-09eb
CTTTAGCTATGCCATGGACCGATTCTTTTTCAGGAAGAGGCTGTTCCAGATGATTAATGA
GCTGCCAACCATTTTCGAAGTTGTGAGCGGCAATGCAAAGCAGTCCAAGGATCTATCTGT
TAACAACAATAATAGCAAAAGCAAACCTAGTGGCGTCAAGGTATCTCTTCTGTTTCACCA
TTTTGTTTTATCAATCTCTGCTAAGTTTTATCTCTTTGTATTCCTCACAAACAGTCTCGC
CAATCCGAATCTCTCTCAAAGGTTGCAAAGATGTCATCTCCACCGCCAAAAGAAGAAGAA
GAAGAAGAAGATGAGAGTGAAGATGAGAGTGAAGACGATGAACAAGGTGCGGTTTGTGGT
GCTTGTGGAGACAACTACGGTACAGATGAGTTCTGGATTTGCTGCGACGCGTGTGAGAAA
TGGTTCCATGGAAAATGTGTGAAGATAACACCTGCAAAAGCAGAACACATCAAGCATTAC
AAATGCCCAACTTGCAGTAACAAGAGAAAAAGAAAGAGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 13-K110358-0022-222-D11C-8409
GenBank Accession LN492088 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110358-0022-222-D11C-8409
ATTTACTCTTTCTTTTTCTCTTGTTACTGCAAGTTGGGCATTTGTAACGATAAAGGCGCT
CTGCTTTTGCAGGTGTTATCTTCACACATCCTTTAAGGAANCATTTCTCACACGCGTCGC
AGCAAATCCAGAACTCATCTGTACCGTAGTTGTCTCCACAAGCACCTCAAACCGCACCTT
GTTCATCGTCTTCACTCTCATCTTCACTCTCATCTTCTTCTTCTTCTTCTTCTTTTGGCG
GTGGAGATGACATCTTTGCAACCTTTGAGAGAGATTCGGATTGGCGAGACTGTTTGTGAG
GAATACAAAGAGATAAAACTTAGCAGAGATTGATAAAACAAAATGGTGAAACAGAAGAGA
TACCTTGACGCCACTAGGTTTGCTTTTGCTATTATTGTTGTTAACAGATAGATCCTTGGA
CTGCTTTGCATTGCCGCTCACAACTTCGAAAATGGTTGGCAGCTCATTAATCATCTGGAA
CAGCCTCTTCCTGAAAAAGAATCGGTCCATGGCATAAGCTTAAAGGCAAGTGATAGCTAA
GTTGTTCAAACAAACCTAATGCCAGAGAAGAATTTGAT

Last Updated on 06.12.2018 16:11