Sequences from confirmation amplicon


Confirmation sequences for line 222D11 and hit At3g14040 are shown.
Line ID 222D11
Locus At3g14040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4649576
Primers used for PCR 50qf - 8474, show primer details
   
Sequence Name 41-K115400-0022-222-D11C-50qf
GenBank Accession LN504195 [GenBank]
Sequencing primer gene specific primer, 50qf: 5'-ATGTATGATCGAGGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115400-0022-222-D11C-50qf
AGGGGAGCTGATGGGTCCTCAATTGTTAACTAGTCCATGTATCATGTATGTATGTATATA
TATAGTCCCCATATGATTAATTTGTTGTACATAACAATGGCTCCGTTGGTTCAATGTAAG
CTTAATAAAAAGAAAGCAAAAAGTTAGTATTAGACTATATAAATTTGTCAAAAAAAATAA
ACGTTATTTCATTAAGAGTTCTAAAATTTTATTGAGACACTGACACAGTGTGAAAATGTT
TTGAAGTATCAAACCTTGTCTTGATGAGGCCGGTTCTTTTTCGCCTTCTATTTACCCAAA
ACTATTGAGACTTAAACTTCAAACTAGTAGAATAAAGAGGCAGATTTCTCTCATCAATGT
ATTAATTTGTAGTTGAATTATTCACCATACCTTATCTTTAATATATGATTTTGGTCAAAA
ATCTATAAATATATCTTGAGTGATGCCAAAAAAAAGGGATCGGCCTCCATGAATTGGTCA
TTCTTCTGCCTTGAGCACAATCCTCGGAATTATTTTTAGCTAATGCAAGAGTTTGTGGTT
TTCTATCTCCCTTTGCTCCTCTATAAAACGGACTTTCGAATCATCAGAACAAAACATCAA
TAAACACAAAAACAAAAGAAGAAGAGAATAAACAAAAGAAGAAAAAAAAAGTATTAAAAC
AAAATCAATAAAAAGAGAATAAAAAATGGTGGGTTCTCACAAAGCAAGCGGAGTGCTTCT
TGTGCTACTAGTGGTTATGGCCACCACAATAGCAAACGGGACACCTGTTGTCGATACAGC
AAAAAATGCAGCTACAGCAGTTGAAGATACAGCAAAAAATGCAGCTACNGCAGTTGGCGG
TGCAGCTGCATCAGTTGGTGCTAAAGTAACAGGTGCCAAACCAGGCGGTGCATCACTTGA
TGTTAAAGCATCAGGAGCCAAAGGAGACGGCAAAACTGATGATAGTGCGGTATGTACCAA
ATGTTTGTTGTAAATTTT
   
Sequence Name 17-K115399-0022-222-D11C-8409
GenBank Accession LN493983 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K115399-0022-222-D11C-8409
CATTGATAACGTTTTTCTTTTATTATTGCTGATCCATATTGTATATTTCCATGTAGATTT
GCCGGTTTTGGCGCAGTCATTGGCCTTCCAAGCGAGGGACCCTTGACCGTCAAAAATAGC
TTTGTTTCCATTCAAAGTGAAATCAGCGAGATTTTCGAAATCAATCCATCCGGCATGAGG
CTTAGTGGTCTTGACCGTAGCCGGAGCCTTAAAATTGCCATTCAATTCCAAAGTGACTGG
ACCTTTGCATGGACCTTTGAACTCTAGGCTCTCTACCAAATACTCACCTTTTGGCACTGT
AATCGTGCTCCCTGCTGCACAAGCTTCTTTCCATGCAGCCGCAAATGCCTATACATGCAT
GCATGAATCAACGTGAAATTCTTTCTTAAACAATACAAAATCGAAATATAATATAAAATA
TACAACAAACATTTGGTACATACCGCACTATCATCAGTTTTGCCGTCTCCTTTGGCTCCT
GATGCTTTAACATCAAGTGATGCACCGCCTGGTTTGGCACCTGTTACTTTAGCACCAACT
GATGCAGCTGCACCGCCAACTGCTGTAGCTGCATTTTTTGCTGTATCTTCAACTGCTGTA
GCTGCATTTTTTGCTGTATCGACAACAGGTGTCCCGTTTGCTATTGTGGTGGCCATAACC
ACTAGTAGCACAAGAAGCACTCCGCTTGCTTTGTGAGAACCCACCATTTTTTATTCTCTT
TTTATTGATTTTGTTTTAATACTTTTTTTTTCTTCTTTTGTTTATTCTCTTCTTCTTTTG
TTTTTGTGTTTATTGATGTTTTGTTCTGATGATTCGAAAGTCCGTTTTATAGAGGAGCAA
AGGGAGATAGAAAACCACAAACTCTTGCATTAGCTAAAAATAATTCCGAGGATTGTGCTC
AAGGCAGAAGAATGACCAATTCATGGAGGCCGATCCCTTTTTT

Last Updated on 06.12.2018 16:11