Sequences from confirmation amplicon


Confirmation sequences for line 223A09 and hit At5g04950 are shown.
Line ID 223A09
Locus At5g04950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1458787
Primers used for PCR MR71 - 8474, show primer details
   
Sequence Name 51-K017366-022-223-A09C-MR71
GenBank Accession LN507342 [GenBank]
Sequencing primer gene specific primer, MR71: 5'-CTTTGACTCTTTGTCCATCCCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K017366-022-223-A09C-MR71
ATTGGTCTACGCCTTCGGTTGCGTTTAGTACGTCCGTTGTGTGGAAGATCATGCGTTTTG
AGAGGTCCGGGTCGCGAGAGACGAGGTTTGAAGCGAGTGTGTTTGCGTGTGAGTCGATGT
CAAAGTTGTGGAACGTCGTGTTGGGGAGGTGAAACTTGGCCAATACGATGGATGTGAGAG
GCATCGGACCCGAACCCACGAAGGCAATCTTGGTGGGGACATGGCTTGAGTGTTGGCTCA
GGAGATCGAACTCGAGCTTGCCTAGCTTGAGGTAGTTGGAGTAGTAAGGAAAGATGTGTA
AATGGTCAAGTGGGTTTTGGTCTTCTTGTAAAGATCCCAAAATTGTGGAGAAGTGTTGCT
CTAAATAACCTTCGGCTTCACCACAAAGCTTGATGAGATTAGCTCTCATGTCTTTGACTT
CTTCACACATATTTGTGACATCGATGTTTGTATCCGTGGGTAAGCACGTGGACACGAGTT
GTCCGAACAAAGTGTCGACATTTTTGGAAGGTTTTAAGCTCTTAAGCTCTTGAGCTTTGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
   
Sequence Name 55-K017366-022-223-A09C-8409
GenBank Accession LN508651 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K017366-022-223-A09C-8409
AGATTTCTCCGATCTAAAGCCATTTACAATTGAATATATCAAAGCTCAAGAGCTTAAGAG
CTTAAAACCTTCCAAAAATGTCGACACTTTGTTCGGACAACTCGTGTCCACGTGCTTACC
CACGGATACAAACATCGATGTCACAAATATGTGTGAAGAAGTCAAAGACATGAGAGCTAA
TCTCATCAAGCTTTGTGGTGAAGCCGAAGGTTATTTAGAGCAACACTTCTCCACAATTTT
GGGATCTTTACAAGAAGACCAAAACCCACTTGACCATTTACACATCTTTCCTTACTACTC
CAACTACCTCAAGCTAGGCAAGCTCGAGTTCGATCTCCTGAGCCAACACTCAAGCCATGT
CCCCACCAAGATTGCCTTCGTGGGTTCGGGTCCGATGCCTCTCACATCCATCGTATTGGC
CAAGTTTCACCTCCCCAACACGACGTTCCACAACTTTGACATCGACTCACACGCAAACAC
ACTCGCTTCAAACCTCGTCTCTCGCGACCCGGACCTCTCAAAACGCATGATCTTCCACAC
AACGGACGTACTAAACGCAACCGAAGGCCTTGACCAATATGACGTCGTTTTCTTAGCGGC
GCTTGTAGGGATGGA

Last Updated on 06.12.2018 16:11