Sequences from confirmation amplicon


Confirmation sequences for line 223C06 and hit At1g62960 are shown.
Line ID 223C06
Locus At1g62960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23318407
Primers used for PCR 17db - 8474, show primer details
   
Sequence Name 48-K114180-0022-223-C06C-17db
GenBank Accession LN506568 [GenBank]
Sequencing primer gene specific primer, 17db: 5'-TTTGACAGAAGATTGAGAGGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114180-0022-223-C06C-17db
ATTGGTTTGATTTGCACAACAGAGAATCCATTAAAGAGACCGAGCATTGCAGAGGTTGTG
CAGGTTCTTGAACTGATCAGAAATGGAATGGAATCATGAAAAGAACTCGTTTATTTTTGC
AGAGAAATTGTGTAGTGTTTACTGTTAAGTTCATTCATTTGTGATTCTTTTGTCTGTTTT
GTTGCTAAAATTGGTAAAAGTTCCATTAACCGGGTCTTCTCTAACCTTAAACCGATTCTG
GATTGATATTATCCGGTTTACTGATATAAAGAGTCTGATTCAGAATAAACCAAATAATCA
AATTGTAATCAATTTTGAGATTTACATGTTTCACAAACTTTTCTAATGCGGTTCATAACT
ACTGGCACATCTCTCTCAGACAAATTACTGAAACAGATACGGAACCATCCTGGTTCGATA
CAGTGACAACAAGATCCTGGTATGAGTATGATGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 12-K114177-0022-223-C06C-8409
GenBank Accession LN491669 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114177-0022-223-C06C-8409
ATCATACTCATACCAGGATCTTGTTGTCACTNTATCGAACCAGGATGGTTCCGTATCTGT
TTCAGTAATTTGTCTGAGAGAGATGTGCCAGTAGTTATGAACCGCATTAGAAAAGTTTGT
GAAACATGTAAATCTCAAAATTGATTACAATTTGATTATTTGGTTTATTCTGAATCAGAC
TCTTTATATCAGTAAACCGGATAATATCAATCCAGAATCGGTTTAAGGTTAGAGAAGACC
CGGTTAATGGAACTTTTACCAATTTTAGCAACAAAACAGACAAAAGAATCACAAATGAAT
GAACTTAACAGTAAACACTACACAATTTCTCTGCAAAAATAAACGAGTTCTTTTCATGAT
TCCATTCCATTTCTGATCAGTTCAAGAACCTGCACAACCTCTGCAATGCTCGGTCTCTTT
AATGGATTCTCTGTTGTGCAAATCAAACCTAATTTCATCACTTGAATCAGCTCATTCTCT
TCAAACCCTCTCAATCTTCTGTCAA

Last Updated on 06.12.2018 16:11