Sequences from confirmation amplicon


Confirmation sequences for line 224A10 and hit At5g41870 are shown.
Line ID 224A10
Locus At5g41870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16759035
Primers used for PCR NR50 - 8474, show primer details
   
Sequence Name 44-K036417-022-224-A10C-NR50
GenBank Accession LN505099 [GenBank]
Sequencing primer gene specific primer, NR50: 5'-TTACTATCCTCGCTCCAACTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K036417-022-224-A10C-NR50
GATCCGGTTTTTTTATCAGTTTCTCTCTTCTACTTAATCATGTTGTTACGTTTCAAATCA
TGACTGATGTGTTTACTAATTACCACAGATTCAAGCTCTAACGTCTGTATAGAAGACTCC
TACATATCTACTGGTGACGACCTTGTTGCGGTGAAGAGCGGTTGGGACGAGTATGGCATT
GCATACAACCGTCCAAGCCGTGACATCACCATCCGCCGCATCACTGGATCTTCCCCATTT
GCTGGAATAGCCATCGGAAGCGAAACTTCTGGCGGAATCCAAAATGTAACAGTTGAAAAC
ATCACTCTCTACAATTCTGGTATCGGCATCCACATCAAGACAAACATTGGCCGAGGAGGA
AGCATACAGGGGATAACAATCTCCGGGGTTTACCTAGAAAAAGTCCGGACCGGGATTAAG
ATCTCTGGCGACACCGGAGATCATCCAGACGATAAATTCAACACATCCGCTCTTCCCATA
GTAAGAGGCATAACGATCAAGAACGTATAAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTCACGCCAAAATGGGGGA
   
Sequence Name 36-K036417-022-224-A10C-8409
GenBank Accession LN502132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K036417-022-224-A10C-8409
GAGCCATTTTATACGTTCTTGATCGTTATGCCTCTTACTATGGGAAGAGCGTATGTGTTG
AATTTATCGTCTGGATGATCTCCGGTGTCGCCAGAGATCTTAATCCCGGTCCGGACTTTT
TCTAGGTAAACCCCGGAGATTGTTATCCCCTGTATGCTTCCTCCTCGGCCAATGTTTGTC
TTGATGTGGATGCCGATACCAGAATTGTAGAGAGTGATGTTTTCAACTGTTACATTTTGG
ATTCCGCCAGAAGTTTCGCTTCCGATGGCTATTCCAGCAAATGGGGAAGATCCAGTGATG
CGGCGGATGGTGATGTCACGGCTTGGACGGTTGTATGCAATGCCATACTCGTCCCAACCG
CTCTTCACCGCAACAAGGTCGTCACCAGTAGATATGTAGGAGTCTTCTATACAGACGTTA
GAGCTTGAATCTGTGGTAATTAGTAAACACATCAGTCATGATTTGAAACGTAACAACATG
ATTAAGTAGAAGAGAGAAACTGATAAAAAAACCTGGATCAATTCCATCGGTGTTGTACGA
ATCAGTTGGAGCAGGAATAG

Last Updated on 06.12.2018 16:11